NDLI: Macroendocytosis and endosome processing in snake motor boutons

Content Provider	PubMed Central
Author	Teng, Haibing Lin, Michael Y. Wilkinson, Robert S.
Copyright Year	2007
Abstract	We have examined the processing of endosomes formed by macroendocytosis (ME), or bulk membrane retrieval, in active motor terminal boutons at the snake nerve–muscle synapse. Endocytic probes were imaged at light (FM1–43) and electron (horseradish peroxidase (HRP)) levels over stimulus frequencies representing low, intermediate and high levels of use. Endosomes formed rapidly (1–2 s) at all frequencies, concomitant with clathrin-mediated vesicular endocytosis (CME). Endosomes dissipated rapidly into vesicles (∼10 s). The dissipation rate was not influenced by activity. Many endosomes split into clusters of 2–20 smaller endosomes of varying size. Vesicles budded from these smaller endosomes, from large endosomes that had not undergone fission into smaller ones, and from precursor membrane infoldings that had not yet internalized. In snake, exocytosed vesicular membrane is not competent for reuse until after a delay (> 3 min). We found that time required for endosome processing is not responsible for this delay. Endosome processing might, however, limit availability of some vesicles for release at very high levels of use. Generally, endosome processing paralleled that of vesicles internalized directly from the plasma membrane via CME, regardless of stimulus frequency. There was no evidence for differential recruitment of ME versus CME depending upon level of use.
Related Links	http://dx.doi.org/10.1113/jphysiol.2007.130989
Starting Page	243
File Format	PDF
ISSN	14697793
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 1
Volume Number	582
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2007-07-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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