NDLI: AMD3100 mobilizes hematopoietic stem cells with long-term repopulating capacity in nonhuman primates

Content Provider	PubMed Central
Author	Larochelle, André Krouse, Allen Metzger, Mark Donald, Orlic Donahue, Robert E. Fricker, Simon Bridger, Gary Dunbar, Cynthia E. Peiman, Hematti
Copyright Year	2006
Abstract	AMD3100, a bicyclam antagonist of the chemokine receptor CXCR4, has been shown to induce rapid mobilization of CD34+ hematopoietic cells in mice, dogs, and humans, offering an alternative to G-CSF mobilization of peripheral-blood hematopoietic stem cells. In this study, AMD3100-mobilized CD34+ cells were phenotypically analyzed, marked with NeoR-containing retroviral vectors, and subsequently transplanted into myeloablated rhesus macaques. We show engraftment of transduced AMD3100-mobilized CD34+ cells with NeoR gene marked myeloid and lymphoid cells up to 32 months after transplantation, demonstrating the ability of AMD3100 to mobilize true long-term repopulating hematopoietic stem cells. More AMD3100-mobilized CD34+ cells are in the G1 phase of the cell cycle and more cells express CXCR4 and VLA-4 compared with G-CSF-mobilized CD34+ cells. In vivo gene marking levels obtained with AMD3100-mobilized CD34+ cells were better than those obtained using CD34+ cells mobilized with G-CSF alone. Overall, these results indicate that AMD3100 mobilizes a population of hematopoietic stem cells with intrinsic characteristics different from those of hematopoietic stem cells mobilized with G-CSF, suggesting fundamental differences in the mechanism of AMD3100-mediated and G-CSF-mediated hematopoietic stem cell mobilization. Thus, AMD3100-mobilized CD34+ cells represent an alternative source of hematopoietic stem cells for clinical stem cell transplantation and genetic manipulation with integrating retroviral vectors.
Related Links	http://dx.doi.org/10.1182/blood-2005-09-3592
Starting Page	3772
File Format	PDF
ISSN	15280020
e-ISSN	15280020
Journal	Blood
Issue Number	9
Volume Number	107
Language	English
Publisher	© 2006 by The American Society of Hematology
Publisher Date	2006-05-01
Access Restriction	Open
Rights Holder	© 2006 by The American Society of Hematology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Hematology Biochemistry Immunology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

BCR-ABL enhances differentiation of long-term repopulating hematopoietic stem cells

Id1, but not Id3, directs long-term repopulating hematopoietic stem-cell maintenance

Hematopoietic stem-cell behavior in nonhuman primates

Defects in osteoblast function but no changes in long-term repopulating potential of hematopoietic stem cells in a mouse chronic inflammatory arthritis model

Overnight transduction with foamyviral vectors restores the long-term repopulating activity of Fancc −/− stem cells

Efficient and stable MGMT-mediated selection of long-term repopulating stem cells in nonhuman primates

The chemokine GROβ mobilizes early hematopoietic stem cells characterized by enhanced homing and engraftment

BIO5192, a small molecule inhibitor of VLA-4, mobilizes hematopoietic stem and progenitor cells

CD105 Is a Surface Marker for Receptor-Targeted Gene Transfer into Human Long-Term Repopulating Hematopoietic Stem Cells

AMD3100 mobilizes hematopoietic stem cells with long-term repopulating capacity in nonhuman primates

Similar Documents

BCR-ABL enhances differentiation of long-term repopulating hematopoietic stem cells

Id1, but not Id3, directs long-term repopulating hematopoietic stem-cell maintenance

Hematopoietic stem-cell behavior in nonhuman primates

Defects in osteoblast function but no changes in long-term repopulating potential of hematopoietic stem cells in a mouse chronic inflammatory arthritis model

Overnight transduction with foamyviral vectors restores the long-term repopulating activity of Fancc −/− stem cells

Efficient and stable MGMT-mediated selection of long-term repopulating stem cells in nonhuman primates

The chemokine GROβ mobilizes early hematopoietic stem cells characterized by enhanced homing and engraftment

BIO5192, a small molecule inhibitor of VLA-4, mobilizes hematopoietic stem and progenitor cells

CD105 Is a Surface Marker for Receptor-Targeted Gene Transfer into Human Long-Term Repopulating Hematopoietic Stem Cells

AMD3100 mobilizes hematopoietic stem cells with long-term repopulating capacity in nonhuman primates