NDLI: Quantitative and morphological analysis of the vascular bed inbronchial biopsy specimens from asthmatic and non-asthmatic subjects

Content Provider	PubMed Central
Author	Salvato, G.
Abstract	BACKGROUND—This studywas designed to establish the number and area (as a percentage) ofbronchial wall vessels in subjects with and without asthma, to obtaininformation on the morphology of the vessels, and to see whetherchanges differed in patients with mild, moderate, and severe asthma. METHODS—Biopsyspecimens were taken using a rigid bronchoscope from the carina of themiddle lobe bronchus of 20 patients with allergic asthma and 20 non-asthmatic controls. Specimens were sectioned and stained withhaematoxylin-eosin, Masson trichrome, PAS, alcian blue-PAS, and orcein.The vessels were counted and the vascular area was calculated asa percentage in the lamina propria, in blind conditions, on PAS stainedsections in 50 microscopic fields (magnification ×1000,0.02 mm2 per field). The vascular area was calculatedusing the points counting procedure (Chalkley point array). Thevascular morphology, intravascular cells, and the perivascular areawere also studied using a magnification up to ×1200. RESULTS—Patientswith asthma had more vessels (mean (SD) 226.70 (74.53)v 172.05 (30.58), p=0.0043) and a largerpercentage vascular area (8.61 (2.38)% v6.81 (2.25)%, p=0.028) than non-asthmatic subjects. Patients withsevere asthma had significantly more vessels than those with mild ormoderate disease (p=0.0044). Asthmatic capillaries and venules hadoedematous walls and thickening of the subendothelial basementmembrane, and hypotrophic or atrophic myocytes and fibrosis in thearterioles. Vessels from asthmatic subjects showed eosinophilrecruitment, activation, and intravascular lysis. Intense eosinophilrecruitment was associated with more marked vascular structuralchanges. Muscular formations protruded into the lumen in the arteriolesof both groups, and in asthmatics these had hypotrophic or atrophicmyocytes and fibrosis. CONCLUSIONS—Morphometricanalysis showed that the bronchial lamina propria of asthmaticsubjects had a larger number of vessels, occupying a larger percentagearea than in non-asthmatic subjects. The number of vessels wascorrelated with the severity of the asthma. Marked alterations to thevascular structure appeared to be associated with intense eosinophilrecruitment and intravascular activation. This is the first reportof asthmatic and non-asthmatic bronchial wall specimens containingintra-arteriolar muscular formations, presumably to regulate blood flowto the capillary network and/or sinusoids. This function might beimpaired when these structures are remodelled in asthmatic patients.
Related Links	http://dx.doi.org/10.1136/thorax.56.12.902
Ending Page	906
Page Count	5
Starting Page	902
File Format	PDF
ISSN	00406376
e-ISSN	14683296
Journal	Thorax
Issue Number	12
Volume Number	56
Language	English
Publisher	BMJ Group
Publisher Date	2001-12-01
Access Restriction	Open
Rights Holder	BMJ Group
Subject Keyword	Pulmonary and Respiratory Medicine Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Pulmonary and Respiratory Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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