NDLI: RNA from the 5′ end of the R2 retrotransposon controls R2 protein binding to and cleavage of its DNA target site

Content Provider	PubMed Central
Author	Christensen, Shawn M. Ye, Junqiang Eickbush, Thomas H.
Copyright Year	2006
Abstract	Non-LTR retrotransposons insert into eukaryotic genomes by target-primed reverse transcription (TPRT), a process in which cleaved DNA targets are used to prime reverse transcription of the element's RNA transcript. Many of the steps in the integration pathway of these elements can be characterized in vitro for the R2 element because of the rigid sequence specificity of R2 for both its DNA target and its RNA template. R2 retrotransposition involves identical subunits of the R2 protein bound to different DNA sequences upstream and downstream of the insertion site. The key determinant regulating which DNA-binding conformation the protein adopts was found to be a 320-nt RNA sequence from near the 5′ end of the R2 element. In the absence of this 5′ RNA the R2 protein binds DNA sequences upstream of the insertion site, cleaves the first DNA strand, and conducts TPRT when RNA containing the 3′ untranslated region of the R2 transcript is present. In the presence of the 320-nt 5′ RNA, the R2 protein binds DNA sequences downstream of the insertion site. Cleavage of the second DNA strand by the downstream subunit does not appear to occur until after the 5′ RNA is removed from this subunit. We postulate that the removal of the 5′ RNA normally occurs during reverse transcription, and thus provides a critical temporal link to first- and second-strand DNA cleavage in the R2 retrotransposition reaction.
Related Links	http://dx.doi.org/10.1073/pnas.0605476103
Ending Page	17607
Page Count	6
Starting Page	17602
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	47
Volume Number	103
Language	English
Publisher	National Academy of Sciences
Publisher Date	2006-11-21
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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