NDLI: Immunoabsorbent nanoparticles based on a tobamovirus displaying protein A

Content Provider	PubMed Central
Author	Werner, Stefan Sylvestre, Marillonnet Hause, Gerd Victor, Klimyuk Gleba, Yuri
Copyright Year	2006
Abstract	Earlier attempts to express peptides longer than 20 aa on the surface of tobamoviruses such as tobacco mosaic virus have failed. Surprisingly, we found that a functional fragment of protein A (133 aa) can be displayed on the surface of a tobamovirus as a C-terminal fusion to the coat protein via a 15-aa linker. The macromolecular nature of these nanoparticles allowed the design of a simple protocol for purification of mAbs with a recovery yield of 50% and >90% product purity. The extremely dense packing of protein A on the nanoparticles (>2,100 copies per viral particle) results in an immunoadsorbent with a binding capacity of 2 g mAb per g. This characteristic, combined with the high level of expression of the nanoparticles (>3 g adsorbent per kg of leaf biomass), provides a very inexpensive self-assembling matrix that could meet the criteria for a single-use industrial immunoadsorbent for antibody purification.
Related Links	http://dx.doi.org/10.1073/pnas.0608869103
Ending Page	17683
Page Count	6
Starting Page	17678
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	47
Volume Number	103
Language	English
Publisher	National Academy of Sciences
Publisher Date	2006-11-21
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Immunoabsorbent nanoparticles based on a tobamovirus displaying protein A.

Production of Immunoabsorbent Nanoparticles by Displaying Single-Domain Protein A on Potato Virus X.

Particle shape enhances specificity of antibody-displaying nanoparticles

TOM1, an Arabidopsis gene required for efficient multiplication of a tobamovirus, encodes a putative transmembrane protein

A cationic host displaying positive cooperativity in water

Antigen-Displaying Lipid-Enveloped PLGA Nanoparticles as Delivery Agents for a Plasmodium vivax Malaria Vaccine

A highly conserved protein family interacting with the fragile X mental retardation protein (FMRP) and displaying selective interactions with FMRP-related proteins FXR1P and FXR2P

Structure-based prediction of protein-protein interactions on a genome-wide scale

Nucleation of protein fibrillation by nanoparticles

Immunoabsorbent nanoparticles based on a tobamovirus displaying protein A

Similar Documents

Immunoabsorbent nanoparticles based on a tobamovirus displaying protein A.

Production of Immunoabsorbent Nanoparticles by Displaying Single-Domain Protein A on Potato Virus X.

Particle shape enhances specificity of antibody-displaying nanoparticles

TOM1, an Arabidopsis gene required for efficient multiplication of a tobamovirus, encodes a putative transmembrane protein

A cationic host displaying positive cooperativity in water

Antigen-Displaying Lipid-Enveloped PLGA Nanoparticles as Delivery Agents for a Plasmodium vivax Malaria Vaccine

A highly conserved protein family interacting with the fragile X mental retardation protein (FMRP) and displaying selective interactions with FMRP-related proteins FXR1P and FXR2P

Structure-based prediction of protein-protein interactions on a genome-wide scale

Nucleation of protein fibrillation by nanoparticles

Immunoabsorbent nanoparticles based on a tobamovirus displaying protein A