NDLI: Lack of nitric oxide- and guanosine 3′:5′-cyclic monophosphate-dependent regulation of α-thrombin-induced calcium transient in endothelial cells of spontaneously hypertensive rat hearts

Content Provider	PubMed Central
Author	Failli, Paola Fazzini, Alessandro Ruocco, Carlo Mazzetti, Luca Cecchi, Enrica Giovannelli, Lisa Marra, Fabio Milani, Stefano Alberto, Giotti
Copyright Year	2000
Abstract	While the expression and/or activity of endothelial nitric oxide synthase (eNOS) has been characterized in spontaneously hypertensive (SHR) and normotensive Wistar Kyoto rat (WKY) hearts, in coronary endothelial cells (ECs) from both strains, the effect of NO on intracellular calcium concentration ([Ca2+]i) is still unknown. Coronary microvascular ECs were isolated from SHR and WKY and characterized. Immunocytochemistry and Western blot analysis showed that eNOS was similarly expressed in ECs from both strains. Measuring [Ca2+]i by imaging analysis of fura-2-loaded cells, we demonstrated that α-thrombin (3−180 U l−1) induced a superimposable dose-dependent calcium transient in ECs from both strains. In WKY ECs, S-nitroso-N-acetyl-DL-penicillamine (SNAP) dose-dependently (10–100 μM) and 0.1 μM atrial natriuretic factor (ANF) reduced the maximum and the decay time of α-thrombin-induced calcium transient. The inhibitory effects of SNAP and ANF were prevented by blocking cyclic GMP-dependent protein kinase. Non selective eNOS inhibitors prolonged the decay time of α-thrombin-induced calcium transient, while the selective inducible NOS inhibitor 1400 W was ineffective. SNAP (100 μM) and 0.1 μM ANF increased cyclic GMP content up to 22.9 and 42.3 fold respectively. In SHR ECs, α-thrombin-induced calcium transient was not modified by SNAP, ANF or eNOS inhibition. SNAP (100 μM) and 0.1 μM ANF increased cyclic GMP content up to 9.3 and 51 fold respectively. In WKY ECs, SNAP dose-dependently (10–100 μM) reduced also bradykinin-induced calcium transient, while in SHR ECs was ineffective. We concluded that in SHR ECs, the cyclic GMP-dependent regulation of calcium transient is lost.
Related Links	http://dx.doi.org/10.1038/sj.bjp.0703453
Ending Page	1476
Page Count	9
Starting Page	1468
File Format	PDF
ISSN	00071188
Journal	British Journal of Pharmacology
Issue Number	7
Volume Number	130
Language	English
Publisher Date	2000-08-01
Access Restriction	Open
Subject Keyword	Pharmacology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Pharmacology

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