NDLI: An agonist-induced switch in G protein coupling of the gonadotropin-releasing hormone receptor regulates pulsatile neuropeptide secretion

Content Provider	PubMed Central
Author	Krsmanovic, Lazar Z. Mores, Nadia Navarro, Carlos E. Arora, Krishan K. Catt, Kevin J.
Copyright Year	2003
Abstract	The pulsatile secretion of gonadotropin-releasing hormone (GnRH) from normal and immortalized hypothalamic GnRH neurons is highly calcium-dependent and is stimulated by cAMP. It is also influenced by agonist activation of the endogenous GnRH receptor (GnRH-R), which couples to Gq/11 as indicated by release of membrane-bound αq/11 subunits and increased inositol phosphate/Ca2+ signaling. Conversely, GnRH antagonists increase membrane-associated αq/11 subunits and abolish pulsatile GnRH secretion. GnRH also stimulates cAMP production but at high concentrations has a pertussis toxin-sensitive inhibitory effect, indicative of receptor coupling to Gi. Coupling of the agonist-activated GnRH-R to both Gs and Gi proteins was demonstrated by the ability of nanomolar GnRH concentrations to reduce membrane-associated αs and αi3 levels and of higher concentrations to diminish αi3 levels. Conversely, αi3 was increased during GnRH antagonist and pertussis toxin treatment, with concomitant loss of pulsatile GnRH secretion. In cholera toxin-treated GnRH neurons, decreases in αs immunoreactivity and increases in cAMP production paralleled the responses to nanomolar GnRH concentrations. Treatment with cholera toxin and 8-bromo-cAMP amplified episodic GnRH pulses but did not affect their frequency. These findings suggest that an agonist concentration-dependent switch in coupling of the GnRH-R between specific G proteins modulates neuronal Ca2+ signaling via Gs-cAMP stimulatory and Gi-cAMP inhibitory mechanisms. Activation of Gi may also inhibit GnRH neuronal function and episodic secretion by regulating membrane ion currents. This autocrine mechanism could serve as a timer to determine the frequency of pulsatile GnRH release by regulating Ca2+- and cAMP-dependent signaling and GnRH neuronal firing.
Related Links	http://dx.doi.org/10.1073/pnas.0535708100
Ending Page	2974
Page Count	6
Starting Page	2969
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	5
Volume Number	100
Language	English
Publisher	The National Academy of Sciences
Publisher Date	2003-03-04
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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