NDLI: Low-Threshold Exocytosis Induced by cAMP-Recruited CaV3.2 (α 1H) Channels in Rat Chromaffin Cells

Content Provider	PubMed Central
Author	Giancippoli, A. Novara, M. Luca, A. De Baldelli, P. Marcantoni, A. Carbone, E. Carabelli, V.
Copyright Year	2006
Abstract	We have studied the functional role of CaV3 channels in triggering fast exocytosis in rat chromaffin cells (RCCs). CaV3 T-type channels were selectively recruited by chronic exposures to cAMP (3 days) via an exchange protein directly activated by cAMP (Epac)-mediated pathway. Here we show that cAMP-treated cells had increased secretory responses, which could be evoked even at very low depolarizations (−50, −40 mV). Potentiation of exocytosis in cAMP-treated cells did not occur in the presence of 50 μM Ni2+, which selectively blocks T-type currents in RCCs. This suggests that the “low-threshold exocytosis” induced by cAMP is due to increased Ca2+ influx through cAMP-recruited T-type channels, rather than to an enhanced secretion downstream of Ca2+ entry, as previously reported for short-term cAMP treatments (20 min). Newly recruited T-type channels increase the fast secretory response at low voltages without altering the size of the immediately releasable pool. They also preserve the Ca2+ dependence of exocytosis, the initial speed of vesicle depletion, and the mean quantal size of single secretory events. All this indicates that cAMP-recruited CaV3 channels enhance the secretory activity of RCCs at low voltages by coupling to the secretory apparatus with a Ca2+ efficacy similar to that of already existing high-threshold Ca2+ channels. Finally, using RT-PCRs we found that the fast inactivating low-threshold Ca2+ current component recruited by cAMP is selectively associated to the α 1H (CaV3.2) channel isoform.
Related Links	http://dx.doi.org/10.1529/biophysj.105.071647
Ending Page	1841
Page Count	12
Starting Page	1830
File Format	PDF
ISSN	00063495
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	5
Volume Number	90
Language	English
Publisher	Biophysical Society
Publisher Date	2006-03-01
Access Restriction	Open
Rights Holder	Biophysical Society
Subject Keyword	Biophysics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

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