NDLI: Vesicle transmembrane potential is required for translocation to the cytosol of externally added FGF-1

Content Provider	PubMed Central
Author	Małecki, Jędrzej Antoni, Więdłocha Wesche, Jørgen Olsnes, Sjur
Copyright Year	2002
Abstract	Externally added fibroblast growth factor-1 (FGF-1) is capable of crossing cellular membranes to reach the cytosol and the nucleus in a number of cell types. We have monitored the translocation of the growth factor by two methods: phosphorylation of FGF-1, and prenylation of an FGF-1 mutant that contains a C-terminal prenylation signal. Inhibition of endosomal acidification by ammonium chloride or monensin did not block the translocation of FGF-1, whereas bafilomycin A1, a specific inhibitor of vacuolar proton pumps, blocked translocation completely. A combination of ionophores expected to dissipate the vesicular membrane potential (valinomycin plus monensin) also fully inhibited the translocation. The inhibition of translocation by bafilomycin A1 was overcome in the presence of monensin or nigericin, while ouabain blocked translocation under these conditions. The data indicate that translocation of FGF-1 to cytosol occurs from the lumen of intracellular vesicles possessing vacuolar proton pumps, and that a vesicular membrane potential is required. Apparently, activation of vesicular Na + /K + -ATPase by monensin or nigericin generates a membrane potential that can support translocation when the proton pump is blocked.
Related Links	http://dx.doi.org/10.1093/emboj/cdf472
Ending Page	4490
Page Count	11
Starting Page	4480
File Format	PDF
ISSN	14602075
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	17
Volume Number	21
Language	English
Publisher	Oxford University Press
Publisher Date	2002-09-02
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

TRAPP stably associates with the Golgi and is required for vesicle docking

Huntingtin is required for ER-to-Golgi transport and for secretory vesicle fusion at the plasma membrane

Myeloid translocation gene 16 is required for maintenance of haematopoietic stem cell quiescence

PACS-1 binding to adaptors is required for acidic cluster motif-mediated protein traffic

Tat is required for efficient HIV-1 reverse transcription.

Ubiquitination and translocation of TRAF2 is required for activation of JNK but not of p38 or NF-κB

From vesicle to cytosol.

G-protein gamma subunit 1 is required for sugar reception in Drosophila

G-protein gamma subunit 1 is required for sugar reception in Drosophila

Vesicle transmembrane potential is required for translocation to the cytosol of externally added FGF-1

Similar Documents

TRAPP stably associates with the Golgi and is required for vesicle docking

Huntingtin is required for ER-to-Golgi transport and for secretory vesicle fusion at the plasma membrane

Myeloid translocation gene 16 is required for maintenance of haematopoietic stem cell quiescence

PACS-1 binding to adaptors is required for acidic cluster motif-mediated protein traffic

Tat is required for efficient HIV-1 reverse transcription.

Ubiquitination and translocation of TRAF2 is required for activation of JNK but not of p38 or NF-κB

From vesicle to cytosol.

G-protein gamma subunit 1 is required for sugar reception in Drosophila

G-protein gamma subunit 1 is required for sugar reception in Drosophila

Vesicle transmembrane potential is required for translocation to the cytosol of externally added FGF-1