NDLI: Mechanism of caveolin filament assembly

Content Provider	PubMed Central
Author	Fernandez, Imma Ying, Yunshu Albanesi, Joseph Anderson, Richard G. W.
Copyright Year	2002
Abstract	Caveolin-1 was the first protein identified that colocalizes with the ≈10-nm filaments found on the inside surface of caveolae membranes. We have used a combination of electron microscopy (EM), circular dichroism, and analytical ultracentrifugation to determine the structure of the oligomers that form when the first 101 aa of caveolin-1 (Cav1–101) are allowed to associate. We determined that amino acids 79–96 in this caveolin-1 fragment are arranged in an α-helix. Cav1–101 oligomers are ≈11 nm in diameter and contain seven molecules of Cav1–101. These subunits, in turn, are able to assemble into 50 nm long × 11 nm diameter filaments that closely match the morphology of the filaments in the caveolae filamentous coat. We propose that the heptameric subunit forms in part through lateral interactions between the α-helices of the seven Cav1–101 units. Caveolin-1, therefore, appears to be the structural molecule of the caveolae filamentous coat.
Related Links	http://dx.doi.org/10.1073/pnas.172196599
Ending Page	11198
Page Count	6
Starting Page	11193
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	17
Volume Number	99
Language	English
Publisher	National Academy of Sciences
Publisher Date	2002-08-20
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Saccharomyces cerevisiae septins: Supramolecular organization of heterooligomers and the mechanism of filament assembly

Mechanism of caveolin filament assembly.

Monitoring intermediate filament assembly by small-angle x-ray scattering reveals the molecular architecture of assembly intermediates

Structure and assembly of P-pili: A protruding hinge region used for assembly of a bacterial adhesion filament

Dynamic assembly of MinD into filament bundles modulated by ATP, phospholipids, and MinE

Insertional assembly of actin filament barbed ends in association with formins produces piconewton forces

Molecular architecture of the Spire–actin nucleus and its implication for actin filament assembly

Assembly mechanism of recombinant spider silk proteins

Skip residues modulate the structural properties of the myosin rod and guide thick filament assembly

Mechanism of caveolin filament assembly

Similar Documents

Saccharomyces cerevisiae septins: Supramolecular organization of heterooligomers and the mechanism of filament assembly

Mechanism of caveolin filament assembly.

Monitoring intermediate filament assembly by small-angle x-ray scattering reveals the molecular architecture of assembly intermediates

Structure and assembly of P-pili: A protruding hinge region used for assembly of a bacterial adhesion filament

Dynamic assembly of MinD into filament bundles modulated by ATP, phospholipids, and MinE

Insertional assembly of actin filament barbed ends in association with formins produces piconewton forces

Molecular architecture of the Spire–actin nucleus and its implication for actin filament assembly

Assembly mechanism of recombinant spider silk proteins

Skip residues modulate the structural properties of the myosin rod and guide thick filament assembly

Mechanism of caveolin filament assembly