NDLI: tRNA-mediated transcription antitermination in vitro: Codon–anticodon pairing independent of the ribosome

Content Provider	PubMed Central
Author	Grundy, Frank J. Winkler, Wade C. Henkin, Tina M.
Copyright Year	2002
Abstract	Uncharged tRNA acts as the effector for transcription antitermination of genes in the T box family in Bacillus subtilis and other Gram-positive bacteria. Genetic studies suggested that expression of these genes is induced by stabilization of an antiterminator element in the leader RNA of each target gene by the cognate uncharged tRNA. The specificity of the tRNA response is dependent on a single codon in the leader, which was postulated to pair with the anticodon of the corresponding tRNA. It was not known whether the leader RNA–tRNA interaction requires additional factors. We show here that tRNA-dependent antitermination occurs in vitro in a purified transcription system, in the absence of ribosomes or accessory factors, demonstrating that the RNA–RNA interaction is sufficient to control gene expression by antitermination. The tRNA response exhibits similar specificity in vivo and in vitro, and the antitermination reaction in vitro is independent of NusA and functions with either B. subtilis or Escherichia coli RNA polymerase.
Related Links	http://dx.doi.org/10.1073/pnas.162366799
Ending Page	11126
Page Count	6
Starting Page	11121
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	17
Volume Number	99
Language	English
Publisher	National Academy of Sciences
Publisher Date	2002-08-20
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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tRNA-mediated transcription antitermination in vitro: codon-anticodon pairing independent of the ribosome.

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