NDLI: CTCF binding and higher order chromatin structure of the H19 locus are maintained in mitotic chromatin

Content Provider	PubMed Central
Author	Burke, Les J. Zhang, Ru Marek, Bartkuhn Tiwari, Vijay K. Tavoosidana, Gholamreza Kurukuti, Sreenivasulu Weth, Christine Leers, Joerg Niels, Galjart Ohlsson, Rolf Rainer, Renkawitz
Copyright Year	2005
Abstract	Most of the transcription factors, RNA polymerases and enhancer binding factors are absent from condensed mitotic chromosomes. In contrast, epigenetic marks of active and inactive genes somehow survive mitosis, since the activity status from one cell generation to the next is maintained. For the zinc-finger protein CTCF, a role in interpreting and propagating epigenetic states and in separating expression domains has been documented. To test whether such a domain structure is preserved during mitosis, we examined whether CTCF is bound to mitotic chromatin. Here we show that in contrast to other zinc-finger proteins, CTCF indeed is bound to mitotic chromosomes. Mitotic binding is mediated by a portion of the zinc-finger DNA binding domain and involves sequence specific binding to target sites. Furthermore, the chromatin loop organized by the CTCF-bound, differentially methylated region at the Igf2/H19 locus can be detected in mitosis. In contrast, the enhancer/promoter loop of the same locus is lost in mitosis. This may provide a novel form of epigenetic memory during cell division.
Related Links	http://dx.doi.org/10.1038/sj.emboj.7600793
Ending Page	3300
Page Count	10
Starting Page	3291
File Format	PDF
ISSN	02614189
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	18
Volume Number	24
Language	English
Publisher Date	2005-08-01
Access Restriction	Open
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

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