NDLI: Novel IκBα Proteolytic Pathway in WEHI231 Immature B Cells

Content Provider	PubMed Central
Author	Miyamoto, Shigeki Seufzer, Bradley J. Shumway, Stuart D.
Copyright Year	1998
Abstract	The Rel/NF-κB family of transcription factors is sequestered in the cytoplasm of most mammalian cells by inhibitor proteins belonging to the IκB family. Degradation of IκB by a phosphorylation-dependent ubiquitin-proteasome (inducible) pathway is believed to allow nuclear transport of active Rel/NF-κB dimers. Rel/NF-κB (a p50–c-Rel dimer) is constitutively nuclear in murine B cells, such as WEHI231 cells. In these cells, p50, c-Rel, and IκBα are synthesized at high levels but only IκBα is rapidly degraded. We have examined the mechanism of IκBα degradation and its relation to constitutive p50–c-Rel activation. We demonstrate that all IκBα is found complexed with c-Rel protein in the cytoplasm. Additionally, rapid IκBα proteolysis is independent of but coexistent with the inducible pathway and can be inhibited by calcium chelators and some calpain inhibitors. Conditions that prevent degradation of IκBα also inhibit nuclear p50–c-Rel activity. Furthermore, the half-life of nuclear c-Rel is much shorter than that of the cytoplasmic form, underscoring the necessity for its continuous nuclear transport to maintain constitutive p50–c-Rel activity. We observed that IκBβ, another NF-κB inhibitor, is also complexed with c-Rel but slowly degraded by a proteasome-dependent process in WEHI231 cells. In addition, IκBβ is basally phosphorylated and cytoplasmic. We thus suggest that calcium-dependent IκBα proteolysis maintains nuclear transport of a p50–c-Rel heterodimer which in turn activates the synthesis of IκBα, p50, and c-Rel to sustain this dynamic process in WEHI231 B cells.
Starting Page	19
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	1
Volume Number	18
Language	English
Publisher	American Society for Microbiology
Publisher Date	1998-01-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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