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Expression, Purification and Sublocalization of SARS-CoV Nucleocapsid Protein in Insect Cells
| Content Provider | Oxford Academic |
|---|---|
| Author | Kong, Yu-Ying Xie, You-Hua Zhang, Yao-Zhou Wang, Yuan Yang, Guan-Zhen Ren, Ai-Xia Wu, Xiang-Fu |
| Description | Abstract. The causative agent of severe acute respiratory syndrome (SARS) is a previously unidentified coronavirus, SARS-CoV. The nucleocapsid (N) protein of S |
| Abstract | The causative agent of severe acute respiratory syndrome (SARS) is a previously unidentified coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is a major viral protein recognized by acute and early convalescent sera from SARS patients. To facilitate the studies on the function and structure of the N protein, this report describe the expression and purification of recombinant SARS-CoV N protein using the baculovirus expression system. Recombinant hexa-histidine-tagged N protein with a molecular mass of 47 kD was produced in insect cells. Recombinant N protein was purified to near homogeneity by Ni2+-NTA affinity chromatography. In addition, we examined the subcellular localization of the N protein by confocal microscopy in Trichoplusia ni BT1 Tn 5B1–4 cells infected with recombinant baculovirus. The N protein was found localized in the cytoplasm as well as in the nucleolus. The purified recombinant N protein can be used in further functional study of SARS-CoV. |
| Related Links | https://academic.oup.com/abbs/article/36/11/754/89 |
| ISSN | 16729145 |
| DOI | 10.1093/abbs/36.11.754 |
| Journal | Acta Biochimica et Biophysica Sinica |
| Volume Number | 36 |
| Language | English |
| Publisher Date | 2004-11-01 |
| Access Restriction | Open |
| Subject Keyword | Severe acute respiratory syndrome (sars) Insect cells Sars coronavirus Nucleocapsid protein Baculovirus |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biochemistry Biophysics |
