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Phenolic Profile, Antioxidant and Enzyme Inhibition Properties of the Chilean Endemic Plant Ovidia pillopillo (Gay) Meissner (Thymelaeaceae)
| Content Provider | MDPI |
|---|---|
| Author | Carmen, Cortés Diego, A. González-Cabrera Beatriz, Sepúlveda Jorge, Bórquez Alfredo, Torres-Benítez Barrientos, Ruth Parra, Claudio Romero-Parra, Javier Pertino, Mariano Walter Areche, Carlos Simirgiotis, Mario J. |
| Copyright Year | 2022 |
| Description | Ovidia pillopillo (Lloime) is an endemic species of the Valdivian Forest of Chile. Little is known on the chemistry and biological activity of this plant. In this study, the phenolic profile, antioxidant capacities and enzyme inhibition capacities (against tyrosinase and cholinesterase) of the plant were investigated for the first time. The phenolic profile of the plant was obtained by UHPLC-MS fingerprinting with high resolution, which showed the presence of several flavonoids and coumarins. The antioxidant potential was measured by FRAP and ORAC (45.56 ± 1.32; 25.33 ± 1.2 μmol Trolox equivalents/g dry plant, respectively) plus ABTS and DPPH methods $(IC_{50}$ = 9.95 ± 0.05 and 6.65 ± 0.5 μg/mL, respectively). Moreover, the flavonoid and phenolic contents were determined (57.33 ± 0.82 and 38.42 ± 1.32, μg of Trolox and quercetin equivalents/100 g dry weight, respectively). The ethanolic extract showed cholinesterase $(IC_{50}$ = 1.94 ± 0.07 and 2.73 ± 0.05 μg/mL, for AChE and BuChE, respectively) and tyrosinase (4.92 ± 0.05 μg/mL) enzyme inhibition activities. Based on these in vitro studies, in silico simulations were performed, which determined that the major compounds as ligands likely docked in the receptors of the enzymes. These results suggest that Ovidia pillopillo produce interesting special coumarins and flavonoids, which are potential candidates for the exploration and preparation of new medicines. |
| Starting Page | 90 |
| e-ISSN | 22181989 |
| DOI | 10.3390/metabo12020090 |
| Journal | Metabolites |
| Issue Number | 2 |
| Volume Number | 12 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-01-18 |
| Access Restriction | Open |
| Subject Keyword | Metabolites Integrative and Complementary Medicine Toxic Plants Daphnetin Coumarins Glycosyl Flavonoids Cholinesterase Inhibition Uhplc–pda–ot-ms/ms Analysis Antioxidants Endemic Species |
| Content Type | Text |
| Resource Type | Article |
