NDLI: Clinical Significance of Circulating Tumor Cell Induced Epithelial-Mesenchymal Transition in Patients with Metastatic Colorectal Cancer by Single-Cell RNA-Sequencing

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Clinical Significance of Circulating Tumor Cell Induced Epithelial-Mesenchymal Transition in Patients with Metastatic Colorectal Cancer by Single-Cell RNA-Sequencing

Content Provider	MDPI
Author	Kozuka, Masahiro Battaglin, Francesca Jayachandran, Priya Wang, Jingyuan Arai, Hiroyuki Soni, Shivani Zhang, Wu Hirai, Mitsuharu Matsusaka, Satoshi Lenz, Heinz-Josef
Copyright Year	2021
Description	Background: Circulating tumor cells (CTCs) are a prognostic marker in patients with metastatic colorectal cancer (mCRC). However, little is known about the characterization of CTCs in mCRC at the single-cell level using RNA sequencing. The purpose of this study was to validate the capability to detect and isolate single CTCs for single-cell RNA sequencing (scRNA-seq) and to identify clinical significance at a single CTC level. Methods: Single CTCs from 27 mCRC patients were collected by CTC-FIND, which is comprised of filter separation and immunomagnetic depletion to collect ultra-pure CTC samples. To address tumor heterogeneity, CTCs were collected without relying on any traditional CTC markers, such as epithelial and mesenchymal cell antigens, and were undertaken by scRNA-seq using SMART-Seq v4. Results: We identified 59 single CTCs which were classified into four groups by epithelial, epithelial-mesenchymal transition (EMT) and stem cell-related gene expression. Patients receiving second or later-line treatment who had EMT gene expressing CTCs had a significantly shorter PFS and OS. Conclusions: Exploiting CTC-FIND with SMART-Seq v4 showed that scRNA-seq of CTCs may shed new insight into tumor heterogeneity of mCRC and that the presence of CTCs expressing EMT-related genes at the single-cell level could have prognostic value in mCRC patients.
Starting Page	4862
e-ISSN	20726694
DOI	10.3390/cancers13194862
Journal	Cancers
Issue Number	19
Volume Number	13
Language	English
Publisher	MDPI
Publisher Date	2021-09-28
Access Restriction	Open
Subject Keyword	Cancers Oncology Circulating Tumor Cell Single-cell Rna-sequencing Epithelial-mesenchymal Transition Colorectal Cancer
Content Type	Text
Resource Type	Article

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