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A Sensitive FRET Biosensor Based on Carbon Dots-Modified Nanoporous Membrane for 8-hydroxy-2′-Deoxyguanosine (8-OHdG) Detection with Au@ZIF-8 Nanoparticles as Signal Quenchers
| Content Provider | MDPI |
|---|---|
| Author | Ye, Weiwei Zhang, Yu Hu, Wei Wang, Liwen Wang, Ping |
| Copyright Year | 2020 |
| Description | A sensitive fluorescence resonance energy transfer (FRET) biosensor is proposed to detect 8-hydroxy-2′-deoxyguanosine (8-OHdG), which is a typical DNA oxidation damage product excreted in human urine. The FRET biosensor was based on carbon dots (CDs)-modified nanoporous alumina membrane with CDs as fluorescence donors. Gold nanoparticles were encapsulated in zeolitic imidazolate framework-8 to form Au@ZIF-8 nanoparticles as signal quenchers. CDs and Au@ZIF-8 nanoparticles were biofunctionalized by 8-OHdG antibody. The capture of 8-OHdG on the membrane substrates can bring Au@ZIF-8 nanoparticles closely to CDs. With 350 nm excitation, the fluorescence of CDs was quenched by Au@ZIF-8 nanoparticles and FRET effect occurred. The quenching efficiency was analyzed. The limit of detection (LOD) was 0.31 nM. Interference experiments of the FRET biosensor showed good specificity for 8-OHdG detection. The biosensor could detect urinary 8-OHdG sensitively and selectively with simple sample pretreatment processes. It shows applicability for detecting biomarkers of DNA damage in urine or other biological fluids. |
| Starting Page | 2044 |
| e-ISSN | 20794991 |
| DOI | 10.3390/nano10102044 |
| Journal | Nanomaterials |
| Issue Number | 10 |
| Volume Number | 10 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2020-10-16 |
| Access Restriction | Open |
| Subject Keyword | Nanomaterials Analytical Chemistry Nanoporous Alumina Membrane Fluorescence Resonance Energy Transfer Au@zif-8 Nanoparticles Dna Oxidation Damage 8-ohdg Detection |
| Content Type | Text |
| Resource Type | Article |
