NDLI: Urinary 8-ohdg ( 8-hydroxy-2 ′-deoxyguanosine ) as Biomarker of Cancer Induced Oxidative Dna Damage

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Urinary 8-ohdg ( 8-hydroxy-2 ′-deoxyguanosine ) as Biomarker of Cancer Induced Oxidative Dna Damage

Content Provider	Semantic Scholar
Author	Raghuwanshi, Deependra Singh
Copyright Year	2017
Abstract	Cancer induced oxidative DNA damage is subject of interest today. The oxidized bases like 8-hydroxy-2’deoxyguanosine are formed due to DNA damage which is continuously excreted through urine. 8-OHdG (8hydroxy-2′ -deoxyguanosine) is one of the major forms of free radical-induced oxidative cuts, and for that reason it has been extensively used as a biomarker for oxidative stress and carcinogenesis. The present study was intended to assess the oxidative DNA damage in lung cancer patients by using a urinary 8-OHdG as biomarker. A case study was carried out on 18 lung cancer patients (12 men and 6 women) who were undergoing chemotherapy. Patients were identified on the basis of various hospital records. Urinary 8-OHdG levels of lung cancer patients were estimated by enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody. Eighteen urine samples of pulmonary cancer patients were collected in and around Nagda town (Ujjain, M.P.India) and were examined for 8-oxo-2′-deoxyguanosine by ELISA technique. The urinary 8-OHdG levels in lung cancer patients were significantly higher than in control (119.48 ± 13.14 ng/ml versus 5782.2 ± 195.3 ng/ml). We conclude that estimation of urinary 8-OHdG is easy and useful biomarker for the diagnosis of cancer induced DNA damage. In our opinion a follow-up of urinary 8-OHdG might be a useful future tool for the evaluation of DNA damage.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://ejpmr.com/admin/download/article/MTUwMTgyNDk4Ni5wZGY=
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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