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Dracocephalum moldavica Ethanol Extract Suppresses LPS-Induced Inflammatory Responses through Inhibition of the JNK/ERK/NF-κB Signaling Pathway and IL-6 Production in RAW 264.7 Macrophages and in Endotoxic-Treated Mice
| Content Provider | MDPI |
|---|---|
| Author | Kim, Kyeong-Min Kim, So-Yeon Mony, Tamanna Jahan Bae, Ho Jung Han, Sang-Deok Lee, Eun-Seok Choi, Seung-Hyuk Hong, Sun Hee Lee, Sang-Deok Park, Se Jin |
| Copyright Year | 2021 |
| Description | The excessive synthesis of interleukin-6 (IL-6) is related to cytokine storm in COVID-19 patients. Moreover, blocking IL-6 has been suggested as a treatment strategy for inflammatory diseases such as sepsis. Sepsis is a severe systemic inflammatory response syndrome with high mortality. In the present study, we investigated the anti-inflammatory and anti-septic effects and the underlying mechanisms of Dracocephalum moldavica ethanol extract (DMEE) on lipopolysaccharide (LPS)-induced inflammatory stimulation in RAW 264.7 macrophages along with septic mouse models. We found that DMEE suppressed the release of inflammatory mediators NO and $PGE_{2}$ and inhibited both the mRNA and protein expression levels of iNOS and COX-2, respectively. In addition, DMEE reduced the release of proinflammatory cytokines, mainly IL-6 and IL-1β, in RAW 264.7 cells by inhibiting the phosphorylation of JNK, ERK and p65. Furthermore, treatment with DMEE increased the survival rate and decreased the level of IL-6 in plasma in LPS-induced septic shock mice. Our findings suggest that DMEE elicits an anti-inflammatory effect in LPS-stimulated RAW 264.7 macrophages and an anti-septic effect on septic mouse model through the inhibition of the ERK/JNK/NF-κB signaling cascades and production of IL-6. |
| Starting Page | 4501 |
| e-ISSN | 20726643 |
| DOI | 10.3390/nu13124501 |
| Journal | Nutrients |
| Issue Number | 12 |
| Volume Number | 13 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-12-16 |
| Access Restriction | Open |
| Subject Keyword | Nutrients Substance Abuse Lipopolysaccharide Inflammation Interleukin-6 Sepsis Dracocephalum Moldavica |
| Content Type | Text |
| Resource Type | Article |
