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The Effect of Visible Light on Cell Envelope Subproteome during Vibrio harveyi Survival at 20 °C in Seawater
| Content Provider | MDPI |
|---|---|
| Author | Maite, Orruño Parada, Claudia Kaberdin, Vladimir Arana, Inés |
| Copyright Year | 2021 |
| Description | A number of Vibrio spp. belong to the well-studied model organisms used to understand the strategies developed by marine bacteria to cope with adverse conditions (starvation, suboptimal temperature, solar radiation, etc.) in their natural environments. Temperature and nutrient availability are considered to be the key factors that influence Vibrio harveyi physiology, morphology, and persistence in aquatic systems. In contrast to the well-studied effects of temperature and starvation on Vibrio survival, little is known about the impact of visible light able to cause photooxidative stress. Here we employ V. harveyi ATCC 14126T as a model organism to analyze and compare the survival patterns and changes in the protein composition of its cell envelope during the long-term permanence of this bacterium in seawater microcosm at 20 °C in the presence and absence of illumination with visible light. We found that V. harveyi exposure to visible light reduces cell culturability likely inducing the entry into the Viable but Non Culturable state (VBNC), whereas populations maintained in darkness remained culturable for at least 21 days. Despite these differences, the starved cells in both populations underwent morphological changes by reducing their size. Moreover, further proteomic analysis revealed a number of changes in the composition of cell envelope potentially accountable for the different adaptation pattern manifested in the absence and presence of visible light. |
| Starting Page | 594 |
| e-ISSN | 20762607 |
| DOI | 10.3390/microorganisms9030594 |
| Journal | Microorganisms |
| Issue Number | 3 |
| Volume Number | 9 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-03-13 |
| Access Restriction | Open |
| Subject Keyword | Microorganisms Biochemical Research Vibrio Seawater Starvation Visible Light Membrane Subproteome |
| Content Type | Text |
| Resource Type | Article |
