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Sensitive Fluorescence Assay for the Detection of Alkaline Phosphatase Based on a $Cu^{2+}$-Thiamine System
| Content Provider | MDPI |
|---|---|
| Author | Zhao, Han Liu, Xinfa Ma, Changbei |
| Copyright Year | 2021 |
| Description | The authors describe a novel, facile, and sensitive fluorometric strategy based on a Cu2+-thiamine (Cu2+-TH) system for the detection of alkaline phosphatase (ALP) activity and inhibition. The principle of the method is as follows. Under a basic conditions, TH, which does not exhibit a fluorescence signal, is oxidized into fluorescent thiochrome (TC) by Cu2+. Ascorbic acid 2-phosphate (AAP), which is the enzyme substrate, is hydrolyzed to produce ascorbic acid (AA) by ALP. The newly formed AA then reduces Cu2+ to Cu+, which prevents the oxidation of TH by Cu2+; as a result, the fluorescent signal becomes weaker. On the contrary, in the absence of ALP, AAP cannot reduce Cu2+; additions of Cu2+ and TH result in a dramatic increase of the fluorescent signal. The sensing strategy displays brilliant sensitivity with a detection limit of 0.08 U/L, and the detection is linear in the concentration range of 0.1 to 100 U/L. This approach was successfully applied to ALP activity in human serum samples, indicating that it is reliable and may be applied to the clinical diagnosis of ALP-related diseases. |
| Starting Page | 674 |
| e-ISSN | 14248220 |
| DOI | 10.3390/s21030674 |
| Journal | Sensors |
| Issue Number | 3 |
| Volume Number | 21 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-01-20 |
| Access Restriction | Open |
| Subject Keyword | Sensors Analytical Chemistry Spectroscopy Alkaline Phosphatase Fluorescence Cu2+-thiamine Inhibitor |
| Content Type | Text |
| Resource Type | Article |
