NDLI: Conventional and Real-Time PCR Targeting $bla_{OXA}$ Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Acinetobacter baumannii Clinical Strains

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Conventional and Real-Time PCR Targeting $bla_{OXA}$ Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Acinetobacter baumannii Clinical Strains

Content Provider	MDPI
Author	Depka, Dagmara Mikucka, Agnieszka Bogiel, Tomasz Rzepka, Mateusz Zawadka, Patryk Gospodarek-Komkowska, Eugenia
Copyright Year	2022
Description	Multidrug-resistant Acinetobacter baumannii, particularly those producing carbapenemases, are spread worldwide. A reliable detection of carbapenemases is essential to choose the appropriate antimicrobial therapy and, consequently, prevent the dissemination of carbapenem-resistant strains. The aim of this study is to examine the molecular basis of the carbapenem resistance mechanism and estimation of conventional PCR and real-time PCR usefulness for the detection of oxacillinases when compared to phenotypic carbapenemases detection. The following methods were evaluated: the CarbAcineto NP test, Carbapenem Inactivation Method, CPO panels of semiautomated antimicrobial susceptibility testing method on the BD Phoenix™ M50 system, conventional Polymerase Chain Reaction and real-time PCR. The $eazyplex^{®}$ SuperBug complete A assay was used as the reference method. Among the tested strains, 39 (67.2%) carried the $bla_{OXA-40}$ gene, while the $bla_{OXA-23}$ gene was noted amongst 19 (32.8%) isolates. The diagnostic sensitivities of the studied assays were as follows: CarbAcineto NP—65.5%; CIM—100%; CPO—100%; conventional PCR—100%; real-time PCR—100%.
Starting Page	455
e-ISSN	20796382
DOI	10.3390/antibiotics11040455
Journal	Antibiotics
Issue Number	4
Volume Number	11
Language	English
Publisher	MDPI
Publisher Date	2022-03-28
Access Restriction	Open
Subject Keyword	Antibiotics Microbiology Acinetobacter Baumannii Carbapenemases Carbapenem-resistant Oxa-like Beta-lactamases Oxa-like Carbapenemases Oxa-23 Oxa-40 Resistance To Carbapenems
Content Type	Text
Resource Type	Article

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