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Antibiotic resistance pattern of Acinetobacter baumannii from burns patients: increase in prevalence of blaOXA-24-like and blaOXA-58-like genes
| Content Provider | Directory of Open Access Journals (DOAJ) |
|---|---|
| Author | Niloofar Tafreshi Laleh Babaeekhou Maryam Ghane |
| Abstract | Background and Objectives: Notwithstanding the increased prevalence of Acinetobacter baumannii drug-resistant isolates, treatment options are progressively limiting. This study aims to provide a recent report on antibiotic susceptibility in burn wound isolates of A. baumannii, and the importance of OXA beta-lactamases in carbapenem resistance. Materials and Methods: The susceptibility levels to different antimicrobial categories were determined among 84 A. baumannii isolates from burn wound infection between 2016 and 2018. Multiplex PCR was used to detect OXA beta-lactamases genes, including blaOXA-51, blaOXA-23, blaOXA-24 and blaOXA-58. ISAba-1 association with blaOXA-51, blaOXA-23 and blaOXA-58 was detected by PCR mapping. Results: All the isolates were determined as multidrug-resistant (MDR) and 69% as extensively drug-resistant (XDR). Different carbapenems MIC ranges (MIC50 and MIC90) were observed among the isolates harboring blaOXA-like genes and isolates with the OXA-24-like enzyme showed higher carbapenems MIC ranges. The prevalence of blaOXA-51-like, blaOXA-23-like, blaOXA-24-like and blaOXA-58-like were 100%, 53.57%, 41.66% and 30.95%, respectively. ISAba-1 insertion sequence was found to be upstream to blaOXA-23-like and blaOXA-58-like genes in 23 out of 45 (71.1%) blaOXA-23-like-positive and 4 out of 23 (15.3) blaOXA-58-like-positive isolates, respectively. Conclusion: Resistance to carbapenems as the last resort for treatment of A. baumannii infections is growing. This study, for the first time in Iran, has observed the increased frequency of blaOXA-24-like and blaOXA-58-like genes and found an association between ISAba-1 and blaOXA-58-like gene, which signifies the possible risk of increased diversity in OXA beta-lactamases and growth in carbapenem resistance. |
| Related Links | https://ijm.tums.ac.ir/index.php/ijm/article/view/2296 |
| ISSN | 20083289 |
| e-ISSN | 20084447 |
| DOI | 10.18502/ijm.v11i6.2222 |
| Journal | Iranian Journal of Microbiology |
| Issue Number | 6 |
| Volume Number | 11 |
| Language | English |
| Publisher | Tehran University of Medical Sciences |
| Publisher Date | 2020-01-01 |
| Publisher Place | Iran |
| Access Restriction | Open |
| Subject Keyword | Microbiology Acinetobacter Baumannii; Antibiotic Susceptibility; Carbapenem; Oxa Beta-lactamases |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology Microbiology (medical) |
