Loading...
Please wait, while we are loading the content...
Similar Documents
Profiling of $N^{6}$-Methyladenosine $(m^{6}$A) Modification Landscape in Response to Drought Stress in Apple (Malus prunifolia (Willd.) Borkh)
| Content Provider | MDPI |
|---|---|
| Author | Mao, Xiushan Hou, Nan Liu, Zhenzhong He, Jieqiang |
| Copyright Year | 2021 |
| Description | Drought stress is a significant environmental factor limiting crop growth worldwide. Malus prunifolia is an important apple species endemic to China and is used for apple cultivars and rootstocks with great drought tolerance. $N^{6}$-methyladenosine $(m^{6}$A) is a common epigenetic modification on messenger RNAs (mRNAs) in eukaryotes which is critical for various biological processes. However, there are no reports on $m^{6}$A methylation in apple response to drought stress. Here, we assessed the $m^{6}$A landscape of M. prunifolia seedlings in response to drought and analyzed the association between $m^{6}$A modification and transcript expression. In total, we found 19,783 and 19,609 significant $m^{6}$A peaks in the control and drought treatment groups, respectively, and discovered a UGUAH (H: A/U/C) motif. In M. prunifolia, under both control and drought conditions, peaks were highly enriched in the 3′ untranslated region (UTR) and coding sequence (CDS). Among 4204 significant differential $m^{6}$A peaks in drought-treated M. prunifolia compared to control-treated M. prunifolia, 4158 genes with $m^{6}$A modification were identified. Interestingly, a large number of hypermethylated peaks (4069) were stimulated by drought treatment compared to hypomethylation. Among the hypermethylated peak-related genes, 972 and 1238 differentially expressed genes (DEGs) were up- and down-regulated in response to drought, respectively. Gene ontology (GO) analyses of differential $m^{6}$A-modified genes revealed that GO slims related to RNA processing, epigenetic regulation, and stress tolerance were significantly enriched. The $m^{6}$A modification landscape depicted in this study sheds light on the epigenetic regulation of M. prunifolia in response to drought stress and indicates new directions for the breeding of drought-tolerant apple trees. |
| Starting Page | 103 |
| e-ISSN | 22237747 |
| DOI | 10.3390/plants11010103 |
| Journal | Plants |
| Issue Number | 1 |
| Volume Number | 11 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-12-30 |
| Access Restriction | Open |
| Subject Keyword | Plants Horticulture Epitranscriptome Gene Expression M6a Methylation Drought Stress Apple |
| Content Type | Text |
| Resource Type | Article |
