NDLI: Purification and Identification of Novel Xanthine Oxidase Inhibitory Peptides Derived from Round Scad (Decapterus maruadsi) Protein Hydrolysates

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Purification and Identification of Novel Xanthine Oxidase Inhibitory Peptides Derived from Round Scad (Decapterus maruadsi) Protein Hydrolysates

Content Provider	MDPI
Author	Hu, Xiao Zhou, Ya Zhou, Shaobo Chen, Shengjun Wu, Yanyan Li, Laihao Yang, Xianqing
Copyright Year	2021
Description	The objective of the present study was to investigate the xanthine oxidase (XO) inhibitory effects of peptides purified and identified from round scad (Decapterus maruadsi) hydrolysates (RSHs). In this study, RSHs were obtained by using three proteases (neutrase, protamex and alcalase). Among them, the RSHs of 6-h hydrolysis by neutrase displayed the strongest XO inhibitory activity and had an abundance of small peptides (<500 Da). Four novel peptides were purified by immobilized metal affinity chromatography and identified by nano-high-performance liquid chromatography mass/mass spectrometry. Their amino acid sequences were KGFP (447.53 Da), FPSV (448.51 Da), FPFP (506.59 Da) and WPDGR (629.66 Da), respectively. Then the peptides were synthesized to evaluate their XO inhibitory activity. The results indicated that the peptides of both FPSV (5 mM) and FPFP (5 mM) exhibited higher XO inhibitory activity (22.61 ± 1.81% and 20.09 ± 2.41% respectively). Fluorescence spectra assay demonstrated that the fluorescence quenching mechanism of XO by these inhibitors (FPSV and FPFP) was a static quenching procedure. The study of inhibition kinetics suggested that the inhibition of both FPSV and FPFP was reversible, and the type of their inhibition was a mixed one. Molecular docking revealed the importance of π-π stacking between Phe residue (contained in peptides) and $Phe^{914}$ (contained in the XO) in the XO inhibitory activity of the peptides.
Starting Page	538
e-ISSN	16603397
DOI	10.3390/md19100538
Journal	Marine drugs
Issue Number	10
Volume Number	19
Language	English
Publisher	MDPI
Publisher Date	2021-09-24
Access Restriction	Open
Subject Keyword	Marine drugs Marine Drugs Spectroscopy Round Scad (decapterus Maruadsi) Hydrolysis Peptides Xanthine Oxidase Inhibitory Purification Identification
Content Type	Text
Resource Type	Article

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