NDLI: Bioactive Fraction of Aronia melanocarpa Fruit Inhibits Adipogenic Differentiation of Cultured 3T3-L1 Cells

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Bioactive Fraction of Aronia melanocarpa Fruit Inhibits Adipogenic Differentiation of Cultured 3T3-L1 Cells

Content Provider	MDPI
Author	Lee, Hwa-Young Suh, Kwang Sik Kim, Young Il Jang, Bong-Keun Kim, Bo-Hyung Yim, Sung-Vin
Copyright Year	2021
Description	Obesity is caused by excessive fat cells and the overgrowth of adipocytes and is a major risk factor for several chronic illnesses. Aronia melanocarpa fruit is rich in anthocyanins and polyphenols and has protective effects against various diseases. In this study, we examined the effect of Aronia extract (Aronia bioactive fraction, $ABF^{®}$) on the biomarkers of the adipogenic pathway during adipocyte differentiation of 3T3-L1 cells. Lipid accumulation was verified by Oil Red O staining. mRNA and protein expression of lipoprotein lipase (LPL), CCAAT/enhancer-binding protein α (C/EBPα), peroxisome proliferator-activated receptor γ (PPARγ), fatty acid-binding protein 2 (FABP2), and fatty acid synthase (FAS) were assayed by RT-qPCR and Western blot analyses. Adiponectin and leptin secretion were measured using enzyme-linked immunosorbent assays. $ABF^{®}$ treatment downregulated lipid accumulation based on Oil Red O staining. $ABF^{®}$-treated cells exhibited decreased mRNA and protein expression of LPL, C/EBPα, PPARγ, FABP2, and FAS. Moreover, $ABF^{®}$ treatment significantly increased adiponectin secretion and decreased leptin secretion. In conclusion, $ABF^{®}$ has anti-adipogenic effects on the differentiation of 3T3-L1 cells and may be used as an anti-obesity nutraceutical.
Starting Page	9224
e-ISSN	20763417
DOI	10.3390/app11199224
Journal	Applied Sciences
Issue Number	19
Volume Number	11
Language	English
Publisher	MDPI
Publisher Date	2021-10-03
Access Restriction	Open
Subject Keyword	Applied Sciences Integrative and Complementary Medicine Aronia Melanocarpa Fruit Aronia Bioactive Fraction (abf®) Obesity 3t3-l1 Cells Adipocyte Anti-adipogenesis
Content Type	Text
Resource Type	Article

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