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Matrigel® enhances 3T3-L1 cell differentiation.
| Content Provider | Europe PMC |
|---|---|
| Author | Josan, Chitmandeep Kakar, Sachin Raha, Sandeep |
| Copyright Year | 2021 |
| Abstract | ABSTRACTCulturing cells on bio-gels are believed to provide a more in vivo-like extracellular matrix. 3T3-L1 cells cultured on Matrigel® significantly alteregd their proliferation and differentiation as compared to growth on tissue culture-coated polystyrene surfaces. Growth on a 250-μm thick layer of Matrigel® facilitated the formation of cellular aggregates of 3T3-L1 cells. Differentiation of 3T3-L1 cells cultured on Matrigel® demonstrated increased levels of mRNA levels for key adipogenic transcription factors (PPARγ, C/EBPα, SREBP1), lipogenic markers (FAS, FABP4, LPL, PLIN1) and markers of adipocyte maturity (LEP), compared to cells cultured directly on a polystyrene tissue culture surface. The gene expression of extracellular matrix proteins (FN1, COL1A1, COL4A1, COL6, LAM) was decreased in 3T3-L1 cells cultured on Matrigel®. Furthermore, growth on Matrigel® increased lipid accumulation in 3T3-L1 cells in the presence and absence of rosiglitazone, a thiazolidinedione routinely used to optimize differentiation in these cells. These changes in adipocyte gene expression and lipid accumulation patterns may be a result of the increased cell–cell and cell–ECM interactions occurring on the Matrigel®, a scenario that is more reflective of an in vivo model. Taken together, our data advance the understanding of the value of culturing 3T3-L1 cells on Matrigel®. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC8296963&blobtype=pdf |
| Page Count | 17 |
| ISSN | 21623945 |
| Journal | Adipocyte |
| Volume Number | 10 |
| DOI | 10.1080/21623945.2021.1951985 |
| PubMed Central reference number | PMC8296963 |
| Issue Number | 1 |
| PubMed reference number | 34288778 |
| e-ISSN | 2162397X |
| Language | English |
| Publisher | Taylor & Francis |
| Publisher Date | 2021-12-01 |
| Access Restriction | Open |
| Rights License | This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. |
| Subject Keyword | 3d cell culture adipocyte matrigel adipose tissue adipogenesis extracellular matrix 3t3-l1 cells adipocyte differentiation rosiglitazone |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Histology |
