NDLI: Bioengineering Approaches to Improve In Vitro Performance of Prepubertal Lamb Oocytes

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Bioengineering Approaches to Improve In Vitro Performance of Prepubertal Lamb Oocytes

Content Provider	MDPI
Author	Maria, Dell’Aquila Mastrorocco, Antonella Cacopardo, Ludovica Lamanna, Daniela Temerario, Letizia Brunetti, Giacomina Carluccio, Augusto Robbe, Domenico
Copyright Year	2021
Description	Juvenile in vitro embryo technology (JIVET) provides exciting opportunities in animal reproduction by reducing the generation intervals. Prepubertal oocytes are also relevant models for studies on oncofertility. However, current JIVET efficiency is still unpredictable, and further improvements are needed in order for it to be used on a large-scale level. This study applied bioengineering approaches to recreate: (1) the three-dimensional (3D) structure of the cumulus–oocyte complex (COC), by constructing—via bioprinting technologies—alginate-based microbeads (COC-microbeads) for 3D in vitro maturation (3D-IVM); (2) dynamic IVM conditions, by culturing the COC in a millifluidic bioreactor; and (3) an artificial follicular wall with basal membrane, by adding granulosa cells (GCs) and type I collagen (CI) during bioprinting. The results show that oocyte nuclear and cytoplasmic maturation, as well as blastocyst quality, were improved after 3D-IVM compared to 2D controls. The dynamic 3D-IVM did not enhance oocyte maturation, but it improved oocyte bioenergetics compared with static 3D-IVM. The computational model showed higher oxygen levels in the bioreactor with respect to the static well. Microbead enrichment with GCs and CI improved oocyte maturation and bioenergetics. In conclusion, this study demonstrated that bioengineering approaches that mimic the physiological follicle structure could be valuable tools to improve IVM and JIVET.
Starting Page	1458
e-ISSN	20734409
DOI	10.3390/cells10061458
Journal	Cells
Issue Number	6
Volume Number	10
Language	English
Publisher	MDPI
Publisher Date	2021-06-10
Access Restriction	Open
Subject Keyword	Cells Reproductive Biology Prepubertal Lamb Oocyte Jivet 3d in Vitro Maturation Millifluidic Bioreactor Bioprinting Granulosa Cells Collagen I Oocyte Bioenergetics Computational Fluidodynamic Model Convection–diffusion–reaction Model
Content Type	Text
Resource Type	Article

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