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RNAi by Soaking Aedes aegypti Pupae in dsRNA
| Content Provider | MDPI |
|---|---|
| Author | Arshad, Fiza Sharma, Arvind Lu, Charleen Gulia-Nuss, Monika |
| Copyright Year | 2021 |
| Description | RNA-interference (RNAi) is a standard technique for functional genomics in adult mosquitoes. However, RNAi in immature, aquatic mosquito stages has been challenging. Several studies have shown successful larval RNAi, usually in combination with a carrier molecule. Except for one study in malaria mosquito, Anopheles gambiae, none of the previous studies has explored RNAi in mosquito pupae. Even in the study that used RNAi in pupae, double stranded RNA (dsRNA) was introduced by microinjection. Here, we describe a successful method by soaking pupae in water containing dsRNA without any carrier or osmotic challenge. The knockdown persisted into adulthood. We expect that this simple procedure will be useful in the functional analysis of genes that highly express in pupae or newly emerged adults. |
| Starting Page | 634 |
| e-ISSN | 20754450 |
| DOI | 10.3390/insects12070634 |
| Journal | Insects |
| Issue Number | 7 |
| Volume Number | 12 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-07-13 |
| Access Restriction | Open |
| Subject Keyword | Insects Tropical Medicine Aedes Aegypti Rna-interference Gene-silencing Pupal Rnai |
| Content Type | Text |
| Resource Type | Article |
