NDLI: dsRNA Uptake in Plant Pests and Pathogens: Insights into RNAi-Based Insect and Fungal Control Technology

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dsRNA Uptake in Plant Pests and Pathogens: Insights into RNAi-Based Insect and Fungal Control Technology

Content Provider	MDPI
Author	Wytinck, Nick Manchur, Christopher L. Li, Vivian H. Whyard, Steve Belmonte, Mark F.
Copyright Year	2020
Description	Efforts to develop more environmentally friendly alternatives to traditional broad-spectrum pesticides in agriculture have recently turned to RNA interference (RNAi) technology. With the built-in, sequence-specific knockdown of gene targets following delivery of double-stranded RNA (dsRNA), RNAi offers the promise of controlling pests and pathogens without adversely affecting non-target species. Significant advances in the efficacy of this technology have been observed in a wide range of species, including many insect pests and fungal pathogens. Two different dsRNA application methods are being developed. First, host induced gene silencing (HIGS) harnesses dsRNA production through the thoughtful and precise engineering of transgenic plants and second, spray induced gene silencing (SIGS) that uses surface applications of a topically applied dsRNA molecule. Regardless of the dsRNA delivery method, one aspect that is critical to the success of RNAi is the ability of the target organism to internalize the dsRNA and take advantage of the host RNAi cellular machinery. The efficiency of dsRNA uptake mechanisms varies across species, and in some uptake is negligible, rendering them effectively resistant to this new generation of control technologies. If RNAi-based methods of control are to be used widely, it is critically important to understand the mechanisms underpinning dsRNA uptake. Understanding dsRNA uptake mechanisms will also provide insight into the design and formulation of dsRNAs for improved delivery and provide clues into the development of potential host resistance to these technologies.
Starting Page	1780
e-ISSN	22237747
DOI	10.3390/plants9121780
Journal	Plants
Issue Number	12
Volume Number	9
Language	English
Publisher	MDPI
Publisher Date	2020-12-16
Access Restriction	Open
Subject Keyword	Plants Entomology Clathrin-mediated Endocytosis Double Stranded Rna Dsrna Uptake Fungi Insects Plant Protection Rna Interference Sid Proteins Spray Induced Gene Silencing
Content Type	Text
Resource Type	Article

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