NDLI: Atractylodin Induces Apoptosis and Inhibits the Migration of A549 Lung Cancer Cells by Regulating ROS-Mediated Signaling Pathways

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Atractylodin Induces Apoptosis and Inhibits the Migration of A549 Lung Cancer Cells by Regulating ROS-Mediated Signaling Pathways

Content Provider	MDPI
Author	Zhang, Tong Li, Shu-Mei Li, Yan-Nan Cao, Jing-Long Xue, Hui Wang, Chang Jin, Cheng-Hao
Copyright Year	2022
Description	Atractylodin (ATR) has anticancer effects on some tumor cells by inducing apoptosis, but its mechanism in lung cancer remains unclear. This study investigates the inhibitory effect of ATR on A549 lung cancer cells. Cell viability was detected by the Cell Counting Kit-8 assay, and results showed that ATR could significantly inhibit the proliferation of A549 cells. Apoptosis was detected by Annexin V-FITC/PI staining, and apoptosis rate and mitochondrial membrane potential were detected by flow cytometry. Results showed that the effect of ATR on the apoptosis of A549 cells was negatively correlated with the change in mitochondrial membrane potential. Western blot analysis showed that ATR regulated apoptosis induced by mitogen-activated protein kinase, signal transducer and activator of transcription 3, and nuclear factor kappa B signaling pathways. Analyses of reactive oxygen species (ROS), cell cycle, and cell migration showed that ATR induced intracellular ROS accumulation as an initiation signal to induce cell cycle arrest regulated by the AKT signaling pathway and cell migration inhibition regulated by the Wnt signaling pathway. Results showed that ATR can inhibit cell proliferation, induce cell apoptosis, induce cell cycle arrest, and inhibit the migration of A549 cells (p < 0.05 was considered statistically significant, * p < 0.05, p < 0.01 and * p < 0.001).
Starting Page	2946
e-ISSN	14203049
DOI	10.3390/molecules27092946
Journal	Molecules
Issue Number	9
Volume Number	27
Language	English
Publisher	MDPI
Publisher Date	2022-05-05
Access Restriction	Open
Subject Keyword	Molecules Integrative and Complementary Medicine Atractylodin Lung Cancer Cell Apoptosis Cell Migration Cell Cycle Reactive Oxygen Species
Content Type	Text
Resource Type	Article

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