NDLI: Resistance Profiling and Molecular Characterization of Extended-Spectrum/Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli Isolated from Healthy Broiler Chickens in South Korea

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Resistance Profiling and Molecular Characterization of Extended-Spectrum/Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli Isolated from Healthy Broiler Chickens in South Korea

Content Provider	MDPI
Author	Song, Hyun-Ju Moon, Dong Chan Mechesso, Abraham Fikru Kang, Hee Young Kim, Mi Hyun Choi, Ji-Hyun Kim, Su-Jeong Yoon, Soon-Seek Lim, Suk-Kyung
Copyright Year	2020
Description	We aimed to identify and characterize extended-spectrum β-lactamase (ESBL)-and/or plasmid-mediated AmpC β-lactamase (pAmpC)-producing Escherichia coli isolated from healthy broiler chickens slaughtered for human consumption in Korea. A total of 332 E. coli isolates were identified from 339 cloacal swabs in 2019. More than 90% of the isolates were resistant to multiple antimicrobials. ESBL/pAmpC-production was noted in 14% (46/332) of the isolates. Six of the CTX-M-β-lactamase-producing isolates were found to co-harbor at least one plasmid-mediated quinolone resistance gene. We observed the co-existence of blaCMY-2 and mcr-1 genes in the same isolate for the first time in Korea. Phylogenetic analysis demonstrated that the majority of blaCMY-2-carrying isolates belonged to subgroup D. Conjugation confirmed the transferability of blaCTX-M and blaCMY-2 genes, as well as non-β-lactam resistance traits from 60.9% (28/46) of the ESBL/pAmpC-producing isolates to a recipient E. coli J53. The ISECP, IS903, and orf477 elements were detected in the upstream or downstream regions. The blaCTX-M and blaCMY-2 genes mainly belonged to the IncI1, IncHI2, and/or IncFII plasmids. Additionally, the majority of ESBL/pAmpC-producing isolates exhibited heterogeneous PFGE profiles. This study showed that healthy chickens act as reservoirs of ESBL/pAmpC-producing E. coli that can potentially be transmitted to humans.
Starting Page	1434
e-ISSN	20762607
DOI	10.3390/microorganisms8091434
Journal	Microorganisms
Issue Number	9
Volume Number	8
Language	English
Publisher	MDPI
Publisher Date	2020-09-18
Access Restriction	Open
Subject Keyword	Microorganisms Pharmacology and Pharmacy Broiler Β-lactamase Bla Ctx-m Bla Cmy-2 E. Coli Mcr-1 Plasmids Quinolone
Content Type	Text
Resource Type	Article

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