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The Role of Acetate Kinase in the Human Parasite Entamoeba histolytica
| Content Provider | MDPI |
|---|---|
| Author | Dang, Thanh Angel, Matthew Cho, Jin Nguyen, Diana Ingram-Smith, Cheryl |
| Copyright Year | 2022 |
| Description | The human parasite Entamoeba histolytica, which causes approximately 100 million cases of amoebic dysentery each year, relies on glycolysis as the major source of ATP production from glucose as it lacks a citric acid cycle and oxidative phosphorylation. Ethanol and acetate, the two major glycolytic end products for E. histolytica, are produced at a ratio of 2:1 under anaerobic conditions, creating an imbalance between NADH production and utilization. In this study we investigated the role of acetate kinase (ACK) in acetate production during glycolysis in E. histolytica metabolism. Analysis of intracellular and extracellular metabolites demonstrated that acetate levels were unaffected in an ACK RNAi cell line, but acetyl-CoA levels and the $NAD^{+}$/NADH ratio were significantly elevated. Moreover, we demonstrated that glyceraldehyde 3-phosphate dehydrogenase catalyzes the ACK-dependent conversion of acetaldehyde to acetyl phosphate in E. histolytica. We propose that ACK is not a major contributor to acetate production, but instead provides a mechanism for maintaining the $NAD^{+}$/NADH balance during ethanol production in the extended glycolytic pathway. |
| Ending Page | 159 |
| Page Count | 13 |
| Starting Page | 147 |
| e-ISSN | 26736772 |
| DOI | 10.3390/parasitologia2020014 |
| Journal | Parasitologia |
| Issue Number | 2 |
| Volume Number | 2 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-06-16 |
| Access Restriction | Open |
| Subject Keyword | Parasitologia Immunology Entamoeba Histolytica Acetate Kinase Glycolysis |
| Content Type | Text |
| Resource Type | Article |
