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Queuine Salvaging in the Human Parasite Entamoeba histolytica
| Content Provider | MDPI |
|---|---|
| Author | Sarid, Lotem Sun, Jingjing Chittrakanwong, Jurairat Trebicz-Geffen, Meirav Ye, Jun Dedon, Peter C. Ankri, Serge |
| Copyright Year | 2022 |
| Description | Queuosine (Q) is a naturally occurring modified nucleoside that occurs in the first position of transfer RNA anticodons such as Asp, Asn, His, and Tyr. As eukaryotes lack pathways to synthesize queuine, the Q nucleobase, they must obtain it from their diet or gut microbiota. Previously, we described the effects of queuine on the physiology of the eukaryotic parasite Entamoeba histolytica and characterized the enzyme EhTGT responsible for queuine incorporation into tRNA. At present, it is unknown how E. histolytica salvages queuine from gut bacteria. We used liquid chromatography–mass spectrometry (LC–MS) and N-acryloyl-3-aminophenylboronic acid (APB) PAGE analysis to demonstrate that E. histolytica trophozoites can salvage queuine from Q or E. coli K12 but not from the modified E. coli QueC strain, which cannot produce queuine. We then examined the role of EhD9, a protein with homology to DNA glycosylase, as a queuine salvage enzyme in E. histolytica. We found that glutathione S-transferase (GST)-EhD9 catalyzed the conversion of Q into queuine. Trophozoites silenced for EhD9 expression are impaired in their ability to form Q-tRNA from Q or from E. coli. We also observed that Q or E. coli K12 partially protects control trophozoites from oxidative stress (OS), but not siEhD9 trophozoites. Overall, our data reveal that EhD9 is central for the direct salvaging of queuine from bacteria and for the resistance of the parasite to OS. |
| Starting Page | 2509 |
| e-ISSN | 20734409 |
| DOI | 10.3390/cells11162509 |
| Journal | Cells |
| Issue Number | 16 |
| Volume Number | 11 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-08-12 |
| Access Restriction | Open |
| Subject Keyword | Cells Biochemical Research Queuine Queuosine Trna Modifications Entamoeba Histolytica Oxidative Stress Resistance |
| Content Type | Text |
| Resource Type | Article |
