NDLI: Proteomics Is Analytical Chemistry: Fitness-for-Purpose in the Application of Top-Down and Bottom-Up Analyses

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Proteomics Is Analytical Chemistry: Fitness-for-Purpose in the Application of Top-Down and Bottom-Up Analyses

Content Provider	MDPI
Author	Coorssen, Jens Yergey, Alfred
Abstract	Molecular mechanisms underlying health and disease function at least in part based on the flexibility and fine-tuning afforded by protein isoforms and post-translational modifications. The ability to effectively and consistently resolve these protein species or proteoforms, as well as assess quantitative changes is therefore central to proteomic analyses. Here we discuss the pros and cons of currently available and developing analytical techniques from the perspective of the full spectrum of available tools and their current applications, emphasizing the concept of fitness-for-purpose in experimental design based on consideration of sample size and complexity; this necessarily also addresses analytical reproducibility and its variance. Data quality is considered the primary criterion, and we thus emphasize that the standards of Analytical Chemistry must apply throughout any proteomic analysis.
File Size	1222656
Ending Page	453
Page Count	14
Starting Page	440
File Format	PDF
e-ISSN	22277382
DOI	10.3390/proteomes3040440
Journal	Proteomes
Issue Number	4
Volume Number	3
Language	English
Publisher Date	2015-12-03
Access Restriction	Open
Subject Keyword	2DE Bottom-up Discovery proteomics LC/MS/MS protein species proteoforms shotgun Top-down two-dimensional gel electrophoresis
Content Type	Text
Resource Type	Article

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