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Optimal Method to Stimulate Cytokine Production and Its Use in Immunotoxicity Assessment

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Optimal Method to Stimulate Cytokine Production and Its Use in Immunotoxicity Assessment

Content Provider	MDPI
Author	Ai, Wenchao Li, Haishan Song, Naining Li, Lei Chen, Huiming
Abstract	Activation of lymphocytes can effectively produce a large amount of cytokines. The types of cytokines produced may depend on stimulating reagents and treatments. To find an optimal method to stimulate cytokine production and evaluate its effect on immunotoxicity assessments, the authors analyzed production of IL-2, IL-4, IL-6, IL-10, IL-13, IFN-γ, TNF-α, GM-CSF, RANTES and TGF-β in undiluted rat whole blood culture (incubation for 0, 2, 4, 6, 8 or 10 h) with different concentrations of PMA/ionomycin, PHA, Con A, LPS and PWM. We also evaluated the effects of cyclosporin A and azathioprine on cytokine production. The results revealed a rapid increase of IL-2, IFN-γ, TNF-α, RANTES and TGF-β secretion within 6 h after stimulation with 25 ng/mL PMA and 1 μg/mL ionomycin. The inhibition of these cytokine profiles reflected the effects of immunosuppressants on the immune system. Therefore, the results of this is study recommend the detection of cytokine profiles in undiluted whole blood stimulated 6 h with 25 ng/mL PMA and 1 μg/mL ionomycin as a powerful immunotoxicity assessment method.
File Size	250880
Ending Page	3842
Page Count	9
Starting Page	3834
File Format	PDF
e-ISSN	16604601
DOI	10.3390/ijerph10093834
Journal	International Journal of Environmental Research and Public Health
Issue Number	9
Volume Number	10
Language	English
Publisher Date	2013-08-27
Access Restriction	Open
Subject Keyword	immunotoxicity assessment cytokines whole blood immunosuppressant
Content Type	Text
Resource Type	Article

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Communication Optimal Method to Stimulate Cytokine Production and Its Use in Immunotoxicity Assessment (2013)

Optimal Method to Stimulate Cytokine Production and Its Use in Immunotoxicity Assessment

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Optimal Method to Stimulate Cytokine Production and Its Use in Immunotoxicity Assessment

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