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| Content Provider | MDPI |
|---|---|
| Author | Chen, Bao Peng, Yinghua Wang, Xinhui Li, Zhiman Sun, Yinshi |
| Abstract | Secoiridoid and iridoid glycosides are the main active components of Gentianae radix. In this work, one iridoid and three secoiridoid glycosides from Gentianae radix have been purified by high-speed counter-current chromatography in two runs using different solvent systems. Ethyl acetate–n-butanol–water (2:1:3, v/v/v) was the optimum solvent system to purify ca. 4.36 mg of loganic acid, 3.05 mg of swertiamarin, and 35.66 mg of gentiopicroside with 98.1%, 97.2% and 98.6% purities, respectively, while 31.15 mg of trifloroside with 98.9% purity was separated using hexane–ethyl acetate–methanol–water (1:3:1:3, v/v/v/v). The structures of the glycosides were identified by mass spectrometry and NMR. After separation, the anti-nitric oxide production effects of the compounds on lipopolysaccharide-induced BV-2 murine microglial cells were also evaluated. All of the compounds inhibited the production of nitric oxide in lipopolysaccharide-induced BV-2 cells with high cell viabilities in a concentration-dependent manner, which demonstrated that were able to be used as a nitric oxide inhibitor. |
| File Size | 3978240 |
| File Format | |
| e-ISSN | 14203049 |
| DOI | 10.3390/molecules22112002 |
| Journal | Molecules |
| Issue Number | 11 |
| Volume Number | 22 |
| Language | English |
| Publisher Date | 2017-11-17 |
| Access Restriction | Open |
| Subject Keyword | glycosides Gentianae radix high-speed counter-current chromatography (HSCCC) anti-NO production effects nitric oxide production |
| Content Type | Text |
| Resource Type | Article |
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