NDLI: The Role of Leucine 191 of Escherichia coliUracil DNA Glycosylase in the Formation of a Highly Stable Complex with the Substrate Mimic, Ugi, and in Uracil Excision from the Synthetic Substrates

Content Provider	Journal of Biological Chemistry (JBC)
Author	Handa, Priya Roy, Sudipta Varshney, Umesh
Abstract	Uracil DNA glycosylase (UDG), a highly conserved DNA repair enzyme, initiates the uracil excision repair pathway. Ugi, a bacteriophage-encoded peptide, potently inhibits UDGs by serving as a remarkable substrate mimic. Structure determination of UDGs has identified regions important for the exquisite specificity in the detection and removal of uracils from DNA and in their interaction with Ugi. In this study, we carried out mutational analysis of the Escherichia coli UDG at Leu191 within the187HPSPLS192 motif (DNA intercalation loop). We show that with the decrease in side chain length at position 191, the stability of the UDG-Ugi complexes regresses. Further, while the L191V and L191F mutants were as efficient as the wild type protein, the L191A and L191G mutants retained only 10 and 1% of the enzymatic activity, respectively. Importantly, however, substitution of Leu191with smaller side chains had no effect on the relative efficiencies of uracil excision from the single-stranded and a corresponding double-stranded substrate. Our results suggest that leucine within the HPSPLS motif is crucial for the uracil excision activity of UDG, and it contributes to the formation of a physiologically irreversible complex with Ugi. We also envisage a role for Leu191 in stabilizing the productive enzyme-substrate complex.
Related Links	http://www.jbc.org/content/276/20/17324.abstract
Ending Page	17331
Starting Page	17324
Page Count	8
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	20
Volume Number	276
DOI	10.1074/jbc.M011166200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2001-05-18
Access Restriction	Open
Subject Keyword	Uracil DNA glycosylase (UDG) Wild type (WT) DNA: REPLICATION REPAIR AND RECOMBINATION
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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The Role Of Leucine 191 Of Escherichia Coli Uracil DNA Glycosylase In The Formation Of A Highly Stable Complex With The Substrate Mimic, Ugi, And In Uracil Excision From The Synthetic Substrates

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