NDLI: Identification of a Novel Human Tankyrase through Its Interaction with the Adaptor Protein Grb14

Content Provider	Journal of Biological Chemistry (JBC)
Author	Lyons, Ruth J. Deane, Róisı́n Lynch, Danielle K. Ye, Zheng-Sheng Jeffrey Sanderson, Georgina M. Eyre, Helen J. Sutherland, Grant R. Daly, Roger J.
Abstract	Tankyrase is an ankyrin repeat-containing poly(ADP-ribose) polymerase originally isolated as a binding partner for the telomeric protein TRF1, but recently identified as a mitogen-activated protein kinase substrate implicated in regulation of Golgi vesicle trafficking. In this study, a novel human tankyrase, designated tankyrase 2, was isolated in a yeast two-hybrid screen as a binding partner for the Src homology 2 domain-containing adaptor protein Grb14. Tankyrase 2 is a 130-kDa protein, which lacks the N-terminal histidine/proline/serine-rich region of tankyrase, but contains a corresponding ankyrin repeat region, sterile α motif module, and poly(ADP-ribose) polymerase homology domain. TheTANKYRASE 2 gene localizes to chromosome 10q23.2 and is widely expressed, with mRNA transcripts particularly abundant in skeletal muscle and placenta. Upon subcellular fractionation, both Grb14 and tankyrase 2 associate with the low density microsome fraction, and association of these proteins in vivo can be detected by co-immunoprecipitation analysis. Deletion analyses implicate the N-terminal 110 amino acids of Grb14 and ankyrin repeats 10–19 of tankyrase 2 in mediating this interaction. This study supports a role for the tankyrases in cytoplasmic signal transduction pathways and suggests that vesicle trafficking may be involved in the subcellular localization or signaling function of Grb14.
Related Links	http://www.jbc.org/content/276/20/17172.abstract
Ending Page	17180
Starting Page	17172
Page Count	9
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	20
Volume Number	276
DOI	10.1074/jbc.M009756200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2001-05-18
Access Restriction	Open
Subject Keyword	Src homology (SH) Antibody (Ab) Activation domain (AD) Binding domain (BD) Base pair(s) (bp) Between pleckstrin homology and Src homology 2 (BPS) Cycloheximide (CHX) 4′, 6-diamidine-2-phenylindole (DAPI) Fluorescencein situ hybridization (FISH) Formiminotransferase cyclodeaminase (FTCD) Grb-Mig (GM) Growth factor receptor-bound (Grb) Glutathione S-transferase (GST) High density microsome (HDM) Histidine/proline/serine-rich (HPS) Insulin-like growth factor 1 (receptor) (IGF-1(R)) Insulin receptor (IR) Insulin-responsive aminopeptidase (IRAP) Kilobase(s) (kb) Low density microsome (LDM) Mitogen-activated protein (MAP) Polyacrylamide gel electrophoresis (PAGE) Poly(ADP-ribose) polymerase (PARP) Phosphate-buffered saline (PBS) PSD-95/Dlg/ZO1 (PDZ) Pleckstrin homology (PH) Sterile α motif (SAM) Telomere repeat binding factor (TRF) MECHANISMS OF SIGNAL TRANSDUCTION
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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