NDLI: Prodigiosins as a New Group of H+/Cl−Symporters That Uncouple Proton Translocators

Content Provider	Journal of Biological Chemistry (JBC)
Author	Sato, Tomohiko Konno, Hiroki Tanaka, Yasufumi Kataoka, Takao Nagai, Kazuo Wasserman, Harry H. Ohkuma, Shoji
Abstract	We reported previously (Kataoka, T., Muroi, M., Ohkuma, S., Waritani, T., Magae, J., Takatsuki, A., Kondo, S., Yamasaki, M., and Nagai, K. (1995) FEBS Lett. 359, 53–59) that prodigiosin 25-C uncoupled vacuolar H+-ATPase, inhibited vacuolar acidification, and affected glycoprotein processing. In the present study we show that prodigiosins (prodigiosin, metacycloprodigiosin, and prodigiosin 25-C) inhibit the acidification activity of H+-ATPase chloride dependently, but not membrane potential formation or ATP hydrolysis activity, and suggest that they promote H+/Cl− symport (or OH−/Cl− exchange, in its equivalence) across vesicular membranes. In fact, prodigiosins displayed H+/Cl− symport activity on liposomal membranes. First of all, they decreased the internal pH of liposomes depending on the external chloride, and raised it depending on the internal chloride when external buffer was free from chloride. Second, their effect was electroneutral and not seriously affected by the application of an inside positive membrane potential generated by K+ and valinomycin. Finally, they promoted the uptake of [36Cl] from external buffers with concomitant intraliposomal acidification when external pH was acidic relative to liposome interior. As prodigiosins hardly inhibit the catalytic activity (ATP hydrolysis) unlike well known OH−/Cl− exchangers (for example, tributyltin chloride), they should provide powerful tools for the study of molecular machinery and cellular activities involving transport of protons and/or chloride.
Related Links	http://www.jbc.org/content/273/34/21455.abstract
Ending Page	21462
Starting Page	21455
Page Count	8
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	34
Volume Number	273
DOI	10.1074/jbc.273.34.21455
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1998-08-21
Access Restriction	Open
Subject Keyword	2-(N-morpholino)-ethanesulfonic acid (MES) Dipropylthiodicarbocyanine iodide (diS-C3-(5)) Dithiothreitol (DTT) F-type H+-ATPase (F-ATPase) Fluorescein-labeled dextran (FD) Transmembrane potential (ΨΔ) Transmembrane pH gradient (ΔpH) Bis(3-phenyl-5-oxoisoxazol-4-yl)pentamethine oxonol (oxonol-V) P-type H+-ATPase (P-ATPase) Tetramethylammonium hydroxide (TMAH) Tributyltin chloride (TBT) N,N-bis(2-hydroxyethyl)glycine. (Bicine) MEMBRANES AND BIOENERGETICS
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Thapsigargin and Dimethyl Sulfoxide Activate Medium Pi ↔ HOH Oxygen Exchange Catalyzed by Sarcoplasmic Reticulum Ca2+-ATPase

Regulation and Reversibility of Vacuolar H+-ATPase

Evidence for a Salt Bridge between Transmembrane Segments 5 and 6 of the Yeast Plasma-membrane H+-ATPase

Prodigiosins uncouple lysosomal vacuolar-type ATPase through promotion of H+/Cl- symport.

Alternative Mechanisms of Vacuolar Acidification in H+-ATPase-deficient Yeast

The Vacuolar H+-ATPase of Lemon Fruits Is Regulated by Variable H+/ATP Coupling and Slip

Importance of Stalk Segment S5 for Intramolecular Communication in the Sarcoplasmic Reticulum Ca2+-ATPase

DnaB from Thermus aquaticus Unwinds Forked Duplex DNA with an Asymmetric Tail Length Dependence

Calculation of the pH of Buffer Solution of 2-[N -Morpholino]ethanesulfonic Acid (MES) from 5°C to 55°C

Prodigiosins as a New Group of H+/Cl−Symporters That Uncouple Proton Translocators

Similar Documents

Thapsigargin and Dimethyl Sulfoxide Activate Medium Pi ↔ HOH Oxygen Exchange Catalyzed by Sarcoplasmic Reticulum Ca2+-ATPase

Regulation and Reversibility of Vacuolar H+-ATPase

Evidence for a Salt Bridge between Transmembrane Segments 5 and 6 of the Yeast Plasma-membrane H+-ATPase

Prodigiosins uncouple lysosomal vacuolar-type ATPase through promotion of H+/Cl- symport.

Alternative Mechanisms of Vacuolar Acidification in H+-ATPase-deficient Yeast

The Vacuolar H+-ATPase of Lemon Fruits Is Regulated by Variable H+/ATP Coupling and Slip

Importance of Stalk Segment S5 for Intramolecular Communication in the Sarcoplasmic Reticulum Ca2+-ATPase

DnaB from Thermus aquaticus Unwinds Forked Duplex DNA with an Asymmetric Tail Length Dependence

Calculation of the pH of Buffer Solution of 2-[N -Morpholino]ethanesulfonic Acid (MES) from 5°C to 55°C

Prodigiosins as a New Group of H+/Cl−Symporters That Uncouple Proton Translocators