NDLI: Recombinant Human mRNA Cap Methyltransferase Binds Capping Enzyme/RNA Polymerase IIo Complexes

Content Provider	Journal of Biological Chemistry (JBC)
Author	Pillutla, Renuka C. Yue, Zhenyu Maldonado, Edio Shatkin, Aaron J.
Abstract	Guanine N-7 methylation is an essential step in the formation of the m7GpppN cap structure that is characteristic of eukaryotic mRNA 5′ ends. The terminal 7-methylguanosine is recognized by cap-binding proteins that facilitate key events in gene expression including mRNA processing, transport, and translation. Here we describe the cloning, primary structure, and properties of human RNA (guanine-7-)methyltransferase. Sequence alignment of the 476-amino acid human protein with the corresponding yeast ABD1 enzyme demonstrated the presence of several conserved motifs known to be required for methyltransferase activity. We also identified a Drosophila open reading frame that encodes a putative RNA (guanine-7-)methyltransferase and contains these motifs. Recombinant human methyltransferase transferred a methyl group fromS-adenosylmethionine to GpppG 5′ends, which are formed on RNA polymerase II transcripts by the sequential action of RNA 5′-triphosphatase and guanylyltransferase activities in the bifunctional mammalian capping enzyme. Binding studies demonstrated that the human cap methyltransferase associated with recombinant capping enzyme. Consistent with selective capping of RNA polymerase II transcripts, methyltransferase also formed ternary complexes with capping enzyme and the elongating form of RNA polymerase II.
Related Links	http://www.jbc.org/content/273/34/21443.abstract
Ending Page	21446
Starting Page	21443
Page Count	4
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	34
Volume Number	273
DOI	10.1074/jbc.273.34.21443
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1998-08-21
Access Restriction	Open
Subject Keyword	Polymerase II (pol II) Hyperphosphorylated elongating form of human RNA polymerase II (pol IIo) Human (guanine-7-)methyltransferase (hMet) C-terminal domain (CTD) Capping enzyme (CE) Human CE (HCE) Expressed sequence tag (EST) S-adenosylmethionine (Adomet) Open reading frame (ORF) Rapid amplification of cDNA ends (RACE) Base pair(s) (bp) Polymerase chain reaction (PCR) GlutathioneS-transferase. (GST)
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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