NDLI: Identification of in Vitro Phosphorylation Sites in the Growth Cone Protein SCG10 EFFECT OF PHOSPHORYLATION SITE MUTANTS ON MICROTUBULE-DESTABILIZING ACTIVITY

Content Provider	Journal of Biological Chemistry (JBC)
Author	Antonsson, Bruno Kassel, Daniel B. Paolo, Gilbert Di Lutjens, Robert Riederer, Beat M. Grenningloh, Gabriele
Abstract	SCG10 is a neuron-specific, membrane-associated protein that is highly concentrated in growth cones of developing neurons. Previous studies have suggested that it is a regulator of microtubule dynamics and that it may influence microtubule polymerization in growth cones. Here, we demonstrate that in vivo, SCG10 exists in both phosphorylated and unphosphorylated forms. By two-dimensional gel electrophoresis, two phosphoisoforms were detected in neonatal rat brain. Using in vitrophosphorylated recombinant protein, four phosphorylation sites were identified in the SCG10 sequence. Ser-50 and Ser-97 were the target sites for protein kinase A, Ser-62 and Ser-73 for mitogen-activated protein kinase and Ser-73 for cyclin-dependent kinase. We also show that overexpression of SCG10 induces a disruption of the microtubule network in COS-7 cells. By expressing different phosphorylation site mutants, we have dissected the roles of the individual phosphorylation sites in regulating its microtubule-destabilizing activity. We show that nonphosphorylatable mutants have increased activity, whereas mutants in which phosphorylation is mimicked by serine-to-aspartate substitutions have decreased activity. These data suggest that the microtubule-destabilizing activity of SCG10 is regulated by phosphorylation, and that SCG10 may link signal transduction of growth or guidance cues involving serine/threonine protein kinases to alterations of microtubule dynamics in the growth cone.
Related Links	http://www.jbc.org/content/273/14/8439.abstract
Ending Page	8446
Starting Page	8439
Page Count	8
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	14
Volume Number	273
DOI	10.1074/jbc.273.14.8439
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1998-04-03
Access Restriction	Open
Subject Keyword	CELL BIOLOGY AND METABOLISM
Alternative Title	Identification of in Vitro Phosphorylation Sites in the Growth Cone Protein SCG10
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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