NDLI: Localization of myxobacterial hemagglutinin in the periplasmic space and on the cell surface of Myxococcus xanthus during developmental aggregation.

Content Provider	Journal of Biological Chemistry (JBC)
Author	Nelson, D. R. Cumsky, M. G. Zusman, D. R.
Abstract	During the period of developmental aggregation which precedes fruiting body formation, the bacterium Myxococcus xanthus produces a large amount of a lectin called myxobacterial hemagglutinin (MBHA). Sequential cell washing, osmotic shock, and disruption of developmental cells showed that as much as 90% of the total hemagglutinating activity can be recovered in the wash and shock fractions. Analysis of the wash and shock fluids by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that these fractions are enriched in MBHA. MBHA was detected on the surface of developmental cells but not vegetative cells by immunofluorescent staining procedures. The fluorescence was localized in distinct patches which were usually located at one or both of the cell poles, although patches of fluorescence could also be seen at additional sites as well. The presence of MBHA on the cell surface was also detected by electron microscopy of developmental cells stained with ferritin-conjugated antibody. Most of the cells showed distinct patches of ferritin staining at one or both of the cell poles; nonpolar staining, which was also observed, was always accompanied by membrane protuberances. The amino acid sequence of the NH2 terminus of MBHA was determined and found to be extremely hydrophobic, suggesting that it may function as a nonprocessed signal for transmembrane transport. The site-specific localization of MBHA at the cell poles suggests that it may function in end-to-end cellular interactions during aggregation.
Related Links	http://www.jbc.org/content/256/23/12589.abstract
Ending Page	12595
Starting Page	12589
Page Count	7
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	23
Volume Number	256
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1981-12-10
Access Restriction	Open
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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Purification and characterization of myxobacterial hemagglutinin, a development-specific lectin of Myxococcus xanthus.

Myxobacterial hemagglutinin: A development-specific lectin of Myxococcus xanthus

Synthesis of several membrane proteins during developmental aggregation in Myxococcus xanthus.

Myxobacterial hemagglutinin: a development-specific lectin of Myxococcus xanthus.

Unravelling a diversity of cellular structures and aggregation dynamics during the early development of Myxococcus xanthus

Quantifying Aggregation Dynamics during Myxococcus xanthus Development ▿ †

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Release of a cell surface protein during development of Myxococcus xanthus.

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Localization of myxobacterial hemagglutinin in the periplasmic space and on the cell surface of Myxococcus xanthus during developmental aggregation.

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