NDLI: Purification and characterization of myxobacterial hemagglutinin, a development-specific lectin of Myxococcus xanthus.

Content Provider	Journal of Biological Chemistry (JBC)
Author	Cumsky, M. G. Zusman, D. R.
Abstract	Myxococcus xanthus is a Gram-negative bacterium that has a complex life cycle which includes cellular aggregation and sporulation. During the period of cellular aggregation, a lectin-like protein called myxobacterial hemagglutinin (MBHA) is synthesized. A four-step purification procedure for MBHA is described. It consists of chromatography on DEAE-cellulose, CM-cellulose, and hydroxyapatite, followed by gel filtration on Bio-Gel P-30. This procedure gives good yields of MBHA (40-50%) free of contaminating proteins. The purified protein has been partially characterized. It exists as a monomer in solution with an apparent Mr = 28,000 and an isoelectric point of 8.3. The amino acid composition of MBHA has been determined. It shows a very high content of glycine (19%) as well as aromatic amino acids (9%); it has a low percentage of charged amino acids. No detectable carbohydrate was found in a large sample (50 micrograms) of MBHA. The far-ultraviolet CD spectrum of MBHA indicates a secondary structure which contains very little alpha-helix, 50 +/- 10% beta-sheet, and 50 +/- 10% random coil. MBHA comprises 1-2% of soluble protein of M. xanthus at the time of cellular aggregation. The fact that it is a lectin suggests that it may play a role in cell-cell recognition or adhesion.
Related Links	http://www.jbc.org/content/256/23/12581.abstract
Ending Page	12588
Starting Page	12581
Page Count	8
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	23
Volume Number	256
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1981-12-10
Access Restriction	Open
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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Myxobacterial hemagglutinin: A development-specific lectin of Myxococcus xanthus

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