NDLI: Stimulation of Arginine Transport in Osmotically Shocked Escherichia coli W Cells by Purified Arginine-binding Protein Fractions

Content Provider	Journal of Biological Chemistry (JBC)
Author	Wilson, Onslow H. Holden, Joseph T.
Abstract	Cold osmotic shock treatment of early stationary phase Escherichia coli W cells reduced the capacity for arginine uptake and accumulation by about 25%. DEAE-cellulose chromatography of the shock fluid separated four protein-containing fractions having relatively specific arginine-binding activity. The reduced transport activity of shocked cells was partially restored by two of these purified protein fractions (I and III). The degree of restoration was linear between 5 and 10 µg per ml for Fraction I; above 10 µg per ml there was a marked reduction in the extent of stimulation. For Fraction III restoration of the initial rate (15 sec) was a curvilinear function of the protein concentration from 5 to 40 µg per ml. The degree of restoration of the steady state level of uptake (5 min) hardly changed within this protein concentration range. Fractions I and III have identical dissociation constants for arginine, the values ranging from 0.5 to 1 x 10-6 m.14C-Arginine was not metabolized by concentrated crude shock protein. The material bound by this protein solution and by purified Fractions I and III was found to have the same Rf value as free arginine.Analysis of the intracellular pool radioactivity after exposure of shocked cells to 14C-arginine and to 14C-arginine plus optimal concentrations of Fractions I and III (in the presence of 4 x 10-3 m amineoxyacetic acid) showed that the bulk of the radioactivity (∼90%) remained in arginine, thereby showing that these proteins stimulate the arginine accumulation process. The proteins are specific in binding and stimulating the transport of arginine. They bound little or no lysine and canavanine and did not stimulate the transport of lysine in shocked cells.These data suggest that cold osmotic shock treatment of E. coli W releases proteins which may participate in the accumulation of arginine by these cells, and that these proteins are not capable of functioning in the accumulation of lysine.
Related Links	http://www.jbc.org/content/244/10/2743.abstract
Ending Page	2749
Starting Page	2743
Page Count	7
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	10
Volume Number	244
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1969-05-25
Access Restriction	Open
Subject Keyword	CHEMISTRY AND METABOLISM OF SUBSTANCES OF LOW MOLECULAR WEIGHT
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Stimulation of arginine transport in osmotically shocked Escherichia coli W cells by purified arginine-binding protein fractions.

Arginine Transport and Metabolism in Osmotically Shocked and Unshocked Cells of Escherichia coli W

Dipeptidases in Spheroplasts and Osmotically Shocked Cells Prepared from Escherichia coli K-12

Glycine Uptake in Escherichia coli I. GLYCINE UPTAKE BY WHOLE CELLS OF ESCHERICHIA COLI W+ AND A d-SERINE-RESISTANT MUTANT

Magnesium Transport in Escherichia coli INHIBITION BY COBALTOUS ION

Magnesium Transport in Escherichia coli INTERFERENCE BY MANGANESE WITH MAGNESIUM METABOLISM

Amino Acid Transport Systems in Escherichia coli K12

The Reduction and Restoration of Galactose Transport in Osmotically Shocked Cells of Escherichia coli

Mechanisms of Active Transport in Isolated Membrane Vesicles IV. GALACTOSE TRANSPORT BY ISOLATED MEMBRANE VESICLES FROM ESCHERICHIA COLI

Stimulation of Arginine Transport in Osmotically Shocked Escherichia coli W Cells by Purified Arginine-binding Protein Fractions

Similar Documents

Stimulation of arginine transport in osmotically shocked Escherichia coli W cells by purified arginine-binding protein fractions.

Arginine Transport and Metabolism in Osmotically Shocked and Unshocked Cells of Escherichia coli W

Dipeptidases in Spheroplasts and Osmotically Shocked Cells Prepared from Escherichia coli K-12

Glycine Uptake in Escherichia coli I. GLYCINE UPTAKE BY WHOLE CELLS OF ESCHERICHIA COLI W+ AND A d-SERINE-RESISTANT MUTANT

Magnesium Transport in Escherichia coli INHIBITION BY COBALTOUS ION

Magnesium Transport in Escherichia coli INTERFERENCE BY MANGANESE WITH MAGNESIUM METABOLISM

Amino Acid Transport Systems in Escherichia coli K12

The Reduction and Restoration of Galactose Transport in Osmotically Shocked Cells of Escherichia coli

Mechanisms of Active Transport in Isolated Membrane Vesicles IV. GALACTOSE TRANSPORT BY ISOLATED MEMBRANE VESICLES FROM ESCHERICHIA COLI

Stimulation of Arginine Transport in Osmotically Shocked Escherichia coli W Cells by Purified Arginine-binding Protein Fractions