NDLI: Formation of Chloroplast Membranes in Chlamydomonas reinhardi y-1 EFFECTS OF INHIBITORS OF PROTEIN SYNTHESIS

Content Provider	Journal of Biological Chemistry (JBC)
Author	Hoober, J. Kenneth Siekevitz, P. Palade, G. E.
Abstract	During normal greening of the y-1 mutant of Chlamydomonas reinhardi, photoreductive activities with 2,6-dichloroindophenol (DCI), an assay for Photosystem II, and with methyl red (ascorbate and DCI as electron donors), an assay for Photosystem I, increased in parallel with chlorophyll. Disc membranes formed approximately in parallel with chlorophyll synthesis and fused to form grana.In the presence of 20 µg of chloramphenicol (CAP) per ml, the cells produced chlorophyll and membranes at 90% of the rate of control cells, but photoreductive activities were drastically reduced. Photoreduction of DCI and of methyl red in CAP-treated cells increased at rates only 35% and 50%, respectively, of those in control cells. Also, in CAP-treated cells the discs rarely fused to form grana. Inhibition of these activities was reversible upon removal of CAP.Chlorophyll synthesis and disc formation in cells in the presence of cycloheximide (CHI) at 1 µg per ml occurred at about one-half of the rate of control cells, but photoreductive activity with DCI and disc fusion into grana was the same as in controls on a chlorophyll basis. At concentrations of CHI above 5 µg per ml, chlorophyll synthesis was completely inhibited.The incorporation of 14C-leucine into total protein by whole cells was inhibited maximally 30 to 50% by CAP and maximally 40 to 50% by CHI; the effects of the drugs were additive.The membranes formed in the presence of CAP could be repaired by transferring the cells to a medium containing CHI (10 µg per ml). During 2 hours of subsequent incubation in light, the existing membranes gained activity until, on a chlorophyll basis, they were at least as active as the membranes formed in control cells.These results indicate that products of both the cytoplasmic and the chloroplastic protein-synthesizing systems are required for disc membrane production in the chloroplast of this alga.
Related Links	http://www.jbc.org/content/244/10/2621.abstract
Ending Page	2631
Starting Page	2621
Page Count	11
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	10
Volume Number	244
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1969-05-25
Access Restriction	Open
Subject Keyword	CHEMISTRY AND METABOLISM OF MACROMOLECULES
Alternative Title	Formation of Chloroplast Membranes in Chlamydomonas reinhardi y-1
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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