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| Content Provider | IET Digital Library |
|---|---|
| Author | Wang, Wenjing Zhou, Fengzhen Cheng, Xiyao Su, Zhengding Guo, Huiling |
| Abstract | To effective capture and universal enrichment of His-tagged protein, polyacrylic acid (PAA) brushes were used to encapsulate Fe3O4 nanoparticles, connect NTA, and Ni2+ to prepare magnetic beads. These materials provide many advantages, such as excellent stability, tuneable particle size, and a surface for further functionalisation with biomolecules. His-tagged green fluorescence protein (GFP) was separated efficiently, and the binding capacity of Fe3O4/MPS@PAA/NTA-Ni2+ was 93.4 mg/g. Compared with High-Affinity Ni-NTA Resin and Ni-NTA Magnetic Agarose Beads, Fe3O4/MPS@PAA/NTA-Ni2+ nanocomposites exhibited higher separation efficiency and binding capacity towards His-tagged GFP. Moreover, the selectivity and recyclability of them for the target proteins were maintained well after six cycles. This study would widen the application of PAA in constructing multifunctional nanocomposites for biomedical fields. |
| Starting Page | 67 |
| Ending Page | 72 |
| Page Count | 6 |
| ISSN | 17518741 |
| Volume Number | 14 |
| e-ISSN | 1751875X |
| Issue Number | Issue 1, Feb (2020) |
| Alternate Webpage(s) | https://digital-library.theiet.org/content/journals/iet-nbt/14/1 |
| Alternate Webpage(s) | https://digital-library.theiet.org/content/journals/10.1049/iet-nbt.2019.0271 |
| Journal | IET Nanobiotechnology |
| Publisher Date | 2019-10-09 |
| Access Restriction | Open |
| Rights Holder | © The Institution of Engineering and Technology |
| Subject Keyword | Amorphous And Nanostructured Magnetic Materials Binding Capacity Biochemistry Biomedical Material Biomolecular Interactions Biomolecules Charge Transfer Complexes Encapsulation Fe3O4-Ni High-affinity Ni-NTA Resin His-tagged GFP His-tagged Green Fluorescence Protein Interactions with Radiations at The Biomolecular Level Iron Compound Molecular Biophysics Nanocomposite Nanofabrication Nanomagnetics Nanomedicine Nanoparticle Nanotechnology Application in Biomedicine Nanotube And Nanostructured Material Ni-NTA Magnetic Agarose Beads Nickel Particle Size Physical Chemistry of Biomolecular Solutions And Condensed State Polyacrylic Acid Brushes Polymer Proteins Resins Separation Separation Efficiency Structure of Solid Clusters Target Proteins |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Electronic, Optical and Magnetic Materials Electrical and Electronic Engineering Biotechnology |
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