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| Content Provider | IET Digital Library |
|---|---|
| Author | Das, Ankita Datta, Pallab Chowdhury, Amit Roy Barui, Ananya |
| Abstract | Umbilical cord-derived mesenchymal stem cells (UCDMSC) are attractive candidates for cell-based regenerative medicine. However, they are susceptible to replicative senescence during repetitive passaging for in-vitro expansion and induced senescence in an oxidative, inflammatory microenvironment in vivo. Aim of this study is to investigate if honey-incorporated matrices can be employed to reduce senescence of UCDMSC. Matrices were prepared by electrospinning solutions of honey with poly-vinyl alcohol (PVA). PVA:honey matrices exhibited free radical scavenging activity. Culture of UCDMSC on PVA:honey matrices showed improvement in cell proliferation compared to pure PVA nanofibres. Expression of vimentin indicated that mesenchymal phenotype is preserved after culturing on these matrices. Further, UCDMSC were serially subcultured and cells of two passages (P2 and P6) were evaluated for reactive oxygen species (ROS) load and senescence parameters. P6 cells showed a higher ROS load and β-galactosidase (β-gal) positive senescent cells compared to P2. However, culturing on PVA:honey substrates significantly reduced both ROS and β-gal markers compared to cells on PVA substrates. Honey contains several antioxidant and anti-inflammatory components, which can reduce the ROS-related senescence. Thus, it is concluded that honey containing nanofibres can be effective substrates for stem cell-based wound healing and regenerative medicine. |
| Starting Page | 870 |
| Ending Page | 880 |
| Page Count | 11 |
| ISSN | 17518741 |
| Volume Number | 14 |
| e-ISSN | 1751875X |
| Issue Number | Issue 9, Dec (2020) |
| Alternate Webpage(s) | https://digital-library.theiet.org/content/journals/iet-nbt/14/9 |
| Alternate Webpage(s) | https://digital-library.theiet.org/content/journals/10.1049/iet-nbt.2019.0288 |
| Journal | IET Nanobiotechnology |
| Publisher Date | 2020-10-05 |
| Access Restriction | Open |
| Rights Holder | © The Institution of Engineering and Technology |
| Subject Keyword | Antiinflammatory Component Antioxidant Component Biochemistry Biomedical Material Cell Proliferation Cell-based Regenerative Medicine Cellular Biophysics Electro spinning Electro spinning Solutions Enzymes Free Radical Scavenging Activity Honey Containing Nanofibre Honey-incorporated Matrix Honey-incorporated Nanofibre Induced Senescence Mesenchymal Phenotype Molecular Biophysics Nanofabrication Nanofibre Nanomedicine Nanotechnology Application in Biomedicine P6 Cell Patient Care And Treatment Physical Chemistry of Biomolecular Solutions And Condensed State Poly-vinyl Alcohol Polymer Fibre Pure PVA Nanofibre PVA Substrates PVA:honey Matrix PVA:honey Substrates Reactive Oxygen Species Load Replicative Senescence ROS-related Senescence Senescence Parameter Stem Cell-based Wound Healing UCDMSC Umbilical Cord-derived Mesenchymal Stem Cell Vimentin Expression Wounds Β-gal Markers Β-galactosidase Positive Senescent Cells |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Electronic, Optical and Magnetic Materials Electrical and Electronic Engineering Biotechnology |
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