NDLI: Preparation and characterisation of magnetosomes based drug conjugates for cancer therapy

Content Provider	IET Digital Library
Author	Raguraman, Varalakshmi Suthindhiran, Krishnamurthy
Abstract	The authors report a novel, effective and enhanced method of conjugating anticancer drug, paclitaxel and gallic acid with magnetosomes. Here, anticancer drugs were functionalised with magnetosomes membrane by direct and indirect (via crosslinkers: glutaraldehyde and 3-aminopropyltriethoxysilane) adsorption methods. The prepared magnetosome–drug conjugates were characterised by Fourier transform infrared, zeta potential, field-emission scanning electron microscope and thermogravimetric analysis/differential scanning calorimetry. The drug-loading efficiency and capacity were found to be 87.874% for paclitaxel (MP) and 71.3% for gallic acid (MG), respectively as calculated by ultraviolet spectroscopy and high-performance liquid chromatography. The drug release demonstrated by the diffusion method in phosphate buffer (PBS), showing a prolonged drug release for MP and MG, respectively. The cytotoxicity effect of the MP and MG displayed cytotoxicity of 69.71%, 55.194% against HeLa and MCF-7 cell lines, respectively. The reactive oxygen species, acridine orange and ethidium bromide and 4, 6-diamidino-2-phenylindole staining of the drug conjugates revealed the apoptotic effect of MP and MG. Further, the regulation of tumour suppressor protein, p53 was determined by western blotting which showed an upregulation of p53. Comparatively, the magnetosome–drug conjugates prepared by direct adsorption achieved the best effects on the drug-loading efficiency and the increased percentage of cancer cell mortality and the upregulation of P53. The proposed research ascertains that magnetosomes could be used as effective nanocarriers in cancer therapy.
Starting Page	815
Ending Page	822
Page Count	8
ISSN	17518741
Volume Number	14
e-ISSN	1751875X
Issue Number	Issue 9, Dec (2020)
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/iet-nbt/14/9
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/10.1049/iet-nbt.2020.0082
Journal	IET Nanobiotechnology
Publisher Date	2020-08-13
Access Restriction	Open
Rights Holder	© The Institution of Engineering and Technology
Subject Keyword	3-aminopropyltriethoxysilane 6-diamidino-2-phenylindole Staining Adsorption Anticancer Drugs Apoptotic Effect Biochemistry Biomedical Material Biomolecular Interactions Cancer Cancer Cell Mortality Cancer Therapy Cellular Biophysics Charge Transfer Complexes Chromatography Cytotoxicity Effect Differential Scanning Calorimetry Diffusion Method Direct Adsorption Drug Delivery System Drug-loading Efficiency Drugs Effective Nanocarriers Electrokinetic Effects Enhanced Method Fourier Transform Spectra Gallic Acid Glutaraldehyde High-performance Liquid Chromatography Infrared Spectra Interactions with Radiations at The Biomolecular Level Magnetosomes Based Drug Magnetosomes Membrane MCF-7 Cell Line Method of Nanofabrication And Processing Molecular Biophysics Nanobiotechnology Nanofabrication Nanomedicine Nanoparticle Nanotechnology Application in Biomedicine Nanotube And Nanostructured Material Patient Care And Treatment Phosphate Buffer Saline Physical Chemistry of Biomolecular Solutions And Condensed State Physics of Subcellular Structure Prepared Magnetosome–drug Conjugates Prolonged Drug Release Proteins Scanning Electron Microscopy Structure of Solid Clusters Toxicology Tumours Zeta Potential Field Emission Scanning Electron Microscopy
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Electronic, Optical and Magnetic Materials Electrical and Electronic Engineering Biotechnology

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