NDLI: Towards enabling engineered microbial-electronic systems: RK2-based conjugal transfer system for Shewanella synthetic biology

Content Provider	IET Digital Library
Author	Hajimorad, M. Gralnick, J. A.
Abstract	Synthetic biology has been traditionally associated with electronics through the application of circuit design concepts towards the genetic engineering of microbes. Due to recent advances in the understanding of extracellular electron transfer in the bacterium Shewanella oneidensis (Shewanella), synthetic biology advances now have the potential of being used towards electronics applications. Towards this end, there is a need for tools that enable the systematic optimisation of genetic circuits in Shewanella. With the introduction of an RK2 origin of transfer cassette, we show that a modular plasmid system constructed prior for synthetic biology efforts in the bacterium Escherichia coli (E. coli) can be ported to Shewanella. In the process, it is also shown that different replication origins can be maintained in Shewanella and that multiple-plasmid strains can be realised in the bacterium. The results suggest that parts accumulated from E. coli synthetic biology efforts over the past decade and a half may be able to be ported to Shewanella, enabling the future engineering of systems where microbes interface with electronics (e.g. biosensors).
Starting Page	426
Ending Page	428
Page Count	3
ISSN	00135194
Volume Number	52
e-ISSN	1350911X
Issue Number	Issue 6, Mar (2016)
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/el/52/6
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/10.1049/el.2015.3226
Journal	Electronics Letters
Publisher Date	2016-01-22
Access Restriction	Open
Rights Holder	© The Institution of Engineering and Technology
Subject Keyword	Bacterium Biological Technique Biological Transport Biosensor Cellular And Subcellular Transmembrane Physics Cellular Transport Circuit Design Concepts Electronics Application Escherichia Coli Extracellular Electron Transfer Genetic Circuit Genetic Engineering Genetics Microbe Interface Microbial-electronic System Microorganisms Modular Plasmid System Multiple-plasmid Strains Physics of Subcellular Structure RK2-based Conjugal Transfer System Shewanella Oneidensis Shewanella Synthetic Biology Systematic Optimisation
Content Type	Text
Resource Type	Article
Subject	Electrical and Electronic Engineering

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